1cz0
From Proteopedia
(New page: 200px<br /><applet load="1cz0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cz0, resolution 2.1Å" /> '''INTRON ENCODED HOMING...) |
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| - | [[Image:1cz0.gif|left|200px]]<br /><applet load="1cz0" size=" | + | [[Image:1cz0.gif|left|200px]]<br /><applet load="1cz0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cz0, resolution 2.1Å" /> | caption="1cz0, resolution 2.1Å" /> | ||
'''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION'''<br /> | '''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A novel mechanism of DNA endonucleolytic cleavage has been visualized for | + | A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1CZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with ZN and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1CZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chevalier, B.]] | [[Category: Chevalier, B.]] | ||
| - | [[Category: Flick, K | + | [[Category: Flick, K E.]] |
| - | [[Category: Galburt, E | + | [[Category: Galburt, E A.]] |
| - | [[Category: Jurica, M | + | [[Category: Jurica, M S.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: NA]] | [[Category: NA]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:11:10 2008'' |
Revision as of 10:11, 21 February 2008
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INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
Overview
A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.
About this Structure
1CZ0 is a Single protein structure of sequence from Physarum polycephalum with and as ligands. Full crystallographic information is available from OCA.
Reference
A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547
Page seeded by OCA on Thu Feb 21 12:11:10 2008
