1d3u
From Proteopedia
(New page: 200px<br /><applet load="1d3u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d3u, resolution 2.4Å" /> '''TATA-BINDING PROTEIN/...) |
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| - | [[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size=" | + | [[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d3u, resolution 2.4Å" /> | caption="1d3u, resolution 2.4Å" /> | ||
'''TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI'''<br /> | '''TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Recently the definition of the metazoan RNA polymerase II and archaeal | + | Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription. |
==About this Structure== | ==About this Structure== | ||
| - | 1D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http:// | + | 1D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Korkhin, Y.]] | [[Category: Korkhin, Y.]] | ||
[[Category: Littlefield, O.]] | [[Category: Littlefield, O.]] | ||
| - | [[Category: Sigler, P | + | [[Category: Sigler, P B.]] |
[[Category: hyperthermophile]] | [[Category: hyperthermophile]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:40 2008'' |
Revision as of 10:12, 21 February 2008
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TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI
Overview
Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.
About this Structure
1D3U is a Protein complex structure of sequences from Pyrococcus woesei. Full crystallographic information is available from OCA.
Reference
The structural basis for the oriented assembly of a TBP/TFB/promoter complex., Littlefield O, Korkhin Y, Sigler PB, Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13668-73. PMID:10570130
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