1d5n
From Proteopedia
(New page: 200px<br /><applet load="1d5n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d5n, resolution 1.55Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1d5n.jpg|left|200px]]<br /><applet load="1d5n" size=" | + | [[Image:1d5n.jpg|left|200px]]<br /><applet load="1d5n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d5n, resolution 1.55Å" /> | caption="1d5n, resolution 1.55Å" /> | ||
'''CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K'''<br /> | '''CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Superoxide dismutase protects organisms from potentially damaging oxygen | + | Superoxide dismutase protects organisms from potentially damaging oxygen radicals by catalyzing the disproportionation of superoxide to oxygen and hydrogen peroxide. We report the use of cryogenic temperatures to kinetically capture the sixth ligand bound to the active site of manganese superoxide dismutase (MnSOD). Synchrotron X-ray diffraction data was collected from Escherichia coli MnSOD crystals grown at pH 8.5 and cryocooled to 100 K. Structural refinement to 1.55 A resolution and close inspection of the active site revealed electron density for a sixth ligand that was interpreted to be a hydroxide ligand. The six-coordinate, distorted-octahedral geometry assumed during inhibition by hydroxide is compared to the room temperature, five-coordinate, trigonal bipyramidal active site determined with crystals grown from practically identical conditions. The gateway residues Tyr34, His30 and a tightly bound water molecule are implicated in closing-off the active site and blocking the escape route of the sixth ligand. |
==About this Structure== | ==About this Structure== | ||
| - | 1D5N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http:// | + | 1D5N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D5N OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Superoxide dismutase]] | [[Category: Superoxide dismutase]] | ||
| - | [[Category: Borgstahl, G | + | [[Category: Borgstahl, G E.O.]] |
[[Category: Chehab, R.]] | [[Category: Chehab, R.]] | ||
[[Category: Pokross, M.]] | [[Category: Pokross, M.]] | ||
[[Category: Sekher, A.]] | [[Category: Sekher, A.]] | ||
| - | [[Category: Snell, E | + | [[Category: Snell, E H.]] |
[[Category: MN]] | [[Category: MN]] | ||
[[Category: manganese superoxide dismutase]] | [[Category: manganese superoxide dismutase]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:19 2008'' |
Revision as of 10:13, 21 February 2008
|
CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K
Overview
Superoxide dismutase protects organisms from potentially damaging oxygen radicals by catalyzing the disproportionation of superoxide to oxygen and hydrogen peroxide. We report the use of cryogenic temperatures to kinetically capture the sixth ligand bound to the active site of manganese superoxide dismutase (MnSOD). Synchrotron X-ray diffraction data was collected from Escherichia coli MnSOD crystals grown at pH 8.5 and cryocooled to 100 K. Structural refinement to 1.55 A resolution and close inspection of the active site revealed electron density for a sixth ligand that was interpreted to be a hydroxide ligand. The six-coordinate, distorted-octahedral geometry assumed during inhibition by hydroxide is compared to the room temperature, five-coordinate, trigonal bipyramidal active site determined with crystals grown from practically identical conditions. The gateway residues Tyr34, His30 and a tightly bound water molecule are implicated in closing-off the active site and blocking the escape route of the sixth ligand.
About this Structure
1D5N is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
Reference
Cryo-trapping the six-coordinate, distorted-octahedral active site of manganese superoxide dismutase., Borgstahl GE, Pokross M, Chehab R, Sekher A, Snell EH, J Mol Biol. 2000 Mar 3;296(4):951-9. PMID:10686094
Page seeded by OCA on Thu Feb 21 12:13:19 2008
