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2ze9

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==Overview==
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{{ABSTRACT_10089437}}
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Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crystallized in six crystal forms using the hanging-drop vapour-diffusion method. The type III and V crystals belong to monoclinic and hexagonal systems, respectively. All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P212121. Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100 A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3 A resolution. A total of 11295 independent reflections to 3 A resolution have been collected from a type VI crystal using a conventional X-ray source, and its structural analysis is currently being conducted using isomorphous replacement methods.
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==About this Structure==
==About this Structure==
2ZE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZE9 OCA].
2ZE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZE9 OCA].
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==Reference==
 
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Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces antibioticus., Suzuki A, Kakuno K, Iwasaki Y, Yamane T, Yamane T, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):317-9. Epub 1999, Jan 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089437 10089437]
 
[[Category: Phospholipase D]]
[[Category: Phospholipase D]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Secreted]]
[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 15:11:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:42:55 2008''

Revision as of 15:42, 25 June 2008

Image:2ze9.png

Template:STRUCTURE 2ze9

Crystal structure of H168A mutant of phospholipase D from Streptomyces antibioticus, as a complex with phosphatidylcholine


Template:ABSTRACT 10089437

About this Structure

2ZE9 is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 18:42:55 2008

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