1deq
From Proteopedia
(New page: 200px<br /><applet load="1deq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1deq, resolution 3.5Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:1deq.gif|left|200px]]<br /><applet load="1deq" size=" | + | [[Image:1deq.gif|left|200px]]<br /><applet load="1deq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1deq, resolution 3.5Å" /> | caption="1deq, resolution 3.5Å" /> | ||
'''THE CRYSTAL STRUCTURE OF MODIFIED BOVINE FIBRINOGEN (AT ~4 ANGSTROM RESOLUTION)'''<br /> | '''THE CRYSTAL STRUCTURE OF MODIFIED BOVINE FIBRINOGEN (AT ~4 ANGSTROM RESOLUTION)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Here we report the crystal structure at approximately 4-A resolution of a | + | Here we report the crystal structure at approximately 4-A resolution of a selectively proteolyzed bovine fibrinogen. This key component in hemostasis is an elongated 340-kDa glycoprotein in the plasma that upon activation by thrombin self-assembles to form the fibrin clot. The crystals are unusual because they are made up of end-to-end bonded molecules that form flexible filaments. We have visualized the entire coiled-coil region of the molecule, which has a planar sigmoidal shape. The primary polymerization receptor pockets at the ends of the molecule face the same way throughout the end-to-end bonded filaments, and based on this conformation, we have developed an improved model of the two-stranded protofibril that is the basic building block in fibrin. Near the middle of the coiled-coil region, the plasmin-sensitive segment is a hinge about which the molecule adopts different conformations. This segment also includes the boundary between the three- and four-stranded portions of the coiled coil, indicating the location on the backbone that anchors the extended flexible Aalpha arm. We suggest that a flexible branch point in the molecule may help accommodate variability in the structure of the fibrin clot. |
==About this Structure== | ==About this Structure== | ||
| - | 1DEQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | + | 1DEQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Brown, J | + | [[Category: Brown, J H.]] |
[[Category: Cohen, C.]] | [[Category: Cohen, C.]] | ||
| - | [[Category: Henschen-Edman, A | + | [[Category: Henschen-Edman, A H.]] |
[[Category: Jun, G.]] | [[Category: Jun, G.]] | ||
[[Category: Volkmann, N.]] | [[Category: Volkmann, N.]] | ||
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:54 2008'' |
Revision as of 10:15, 21 February 2008
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THE CRYSTAL STRUCTURE OF MODIFIED BOVINE FIBRINOGEN (AT ~4 ANGSTROM RESOLUTION)
Overview
Here we report the crystal structure at approximately 4-A resolution of a selectively proteolyzed bovine fibrinogen. This key component in hemostasis is an elongated 340-kDa glycoprotein in the plasma that upon activation by thrombin self-assembles to form the fibrin clot. The crystals are unusual because they are made up of end-to-end bonded molecules that form flexible filaments. We have visualized the entire coiled-coil region of the molecule, which has a planar sigmoidal shape. The primary polymerization receptor pockets at the ends of the molecule face the same way throughout the end-to-end bonded filaments, and based on this conformation, we have developed an improved model of the two-stranded protofibril that is the basic building block in fibrin. Near the middle of the coiled-coil region, the plasmin-sensitive segment is a hinge about which the molecule adopts different conformations. This segment also includes the boundary between the three- and four-stranded portions of the coiled coil, indicating the location on the backbone that anchors the extended flexible Aalpha arm. We suggest that a flexible branch point in the molecule may help accommodate variability in the structure of the fibrin clot.
About this Structure
1DEQ is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
The crystal structure of modified bovine fibrinogen., Brown JH, Volkmann N, Jun G, Henschen-Edman AH, Cohen C, Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):85-90. PMID:10618375
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