1dgm
From Proteopedia
(New page: 200px<br /><applet load="1dgm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dgm, resolution 1.80Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1dgm.jpg|left|200px]]<br /><applet load="1dgm" size=" | + | [[Image:1dgm.jpg|left|200px]]<br /><applet load="1dgm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dgm, resolution 1.80Å" /> | caption="1dgm, resolution 1.80Å" /> | ||
'''CRYSTAL STRUCTURE OF ADENOSINE KINASE FROM TOXOPLASMA GONDII'''<br /> | '''CRYSTAL STRUCTURE OF ADENOSINE KINASE FROM TOXOPLASMA GONDII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Human infection with Toxoplasma gondii is an important cause of morbidity | + | Human infection with Toxoplasma gondii is an important cause of morbidity and mortality. Protozoan parasites such as T. gondii are incapable of de novo purine biosynthesis and must acquire purines from their host, so the purine salvage pathway offers a number of potential targets for antiparasitic chemotherapy. In T. gondii tachyzoites, adenosine is the predominantly salvaged purine nucleoside, and thus adenosine kinase is a key enzyme in the purine salvage pathway of this parasite. The structure of T. gondii adenosine kinase was solved using molecular replacement and refined by simulated annealing at 1.8 A resolution to an R-factor of 0.214. The overall structure and the active site geometry are similar to human adenosine kinase, although there are significant differences. The T. gondii adenosine kinase has several unique features compared to the human sequence, including a five-residue deletion in one of the four linking segments between the two domains, which is probably responsible for a major change in the orientation of the two domains with respect to each other. These structural differences suggest the possibility of developing specific inhibitors of the parasitic enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1DGM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with MG, CL, ADN and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] Full crystallographic information is available from [http:// | + | 1DGM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=ADN:'>ADN</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DGM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Toxoplasma gondii]] | [[Category: Toxoplasma gondii]] | ||
[[Category: Chattopadhyay, D.]] | [[Category: Chattopadhyay, D.]] | ||
| - | [[Category: Cook, W | + | [[Category: Cook, W J.]] |
| - | [[Category: DeLucas, L | + | [[Category: DeLucas, L J.]] |
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: ADN]] | [[Category: ADN]] | ||
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[[Category: toxoplasma gondii]] | [[Category: toxoplasma gondii]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:29 2008'' |
Revision as of 10:16, 21 February 2008
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CRYSTAL STRUCTURE OF ADENOSINE KINASE FROM TOXOPLASMA GONDII
Overview
Human infection with Toxoplasma gondii is an important cause of morbidity and mortality. Protozoan parasites such as T. gondii are incapable of de novo purine biosynthesis and must acquire purines from their host, so the purine salvage pathway offers a number of potential targets for antiparasitic chemotherapy. In T. gondii tachyzoites, adenosine is the predominantly salvaged purine nucleoside, and thus adenosine kinase is a key enzyme in the purine salvage pathway of this parasite. The structure of T. gondii adenosine kinase was solved using molecular replacement and refined by simulated annealing at 1.8 A resolution to an R-factor of 0.214. The overall structure and the active site geometry are similar to human adenosine kinase, although there are significant differences. The T. gondii adenosine kinase has several unique features compared to the human sequence, including a five-residue deletion in one of the four linking segments between the two domains, which is probably responsible for a major change in the orientation of the two domains with respect to each other. These structural differences suggest the possibility of developing specific inhibitors of the parasitic enzyme.
About this Structure
1DGM is a Single protein structure of sequence from Toxoplasma gondii with , , and as ligands. Active as Adenosine kinase, with EC number 2.7.1.20 Full crystallographic information is available from OCA.
Reference
Crystal structure of adenosine kinase from Toxoplasma gondii at 1.8 A resolution., Cook WJ, DeLucas LJ, Chattopadhyay D, Protein Sci. 2000 Apr;9(4):704-12. PMID:10794412
Page seeded by OCA on Thu Feb 21 12:16:29 2008
Categories: Adenosine kinase | Single protein | Toxoplasma gondii | Chattopadhyay, D. | Cook, W J. | DeLucas, L J. | ACY | ADN | CL | MG | Purine metabolism
