1di0
From Proteopedia
(New page: 200px<br /><applet load="1di0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1di0, resolution 2.7Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1di0.gif|left|200px]]<br /><applet load="1di0" size=" | + | [[Image:1di0.gif|left|200px]]<br /><applet load="1di0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1di0, resolution 2.7Å" /> | caption="1di0, resolution 2.7Å" /> | ||
'''CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS'''<br /> | '''CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the three-dimensional structure of 6, 7-dimethyl-8-ribityllumazine synthase (lumazine synthase) from Brucella | + | We have determined the three-dimensional structure of 6, 7-dimethyl-8-ribityllumazine synthase (lumazine synthase) from Brucella abortus, the infectious organism of the disease brucellosis in animals. This enzyme catalyses the formation of 6, 7-dimethyl-8-ribityllumazine, the penultimate product in the synthesis of riboflavin. The three-dimensional X-ray crystal structure of the enzyme from B. abortus has been solved and refined at 2.7 A resolution to a final R-value of 0.18 (R(free)=0.23). The macromolecular assembly of the enzyme differs from that of the enzyme from Bacillus subtilis, the only other lumazine synthase structure known. While the protein from B. subtilis assembles into a 60 subunit icosahedral capsid built from 12 pentameric units, the enzyme from B. abortus is pentameric in its crystalline form. Nonetheless, the active sites of the two enzymes are virtually identical indicating inhibitors to theses enzymes could be effective pharmaceuticals across a broad species range. Furthermore, we compare the structures of the enzyme from B. subtilis and B. abortus and describe the C teminus structure which accounts for the differences in quaternary structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1DI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DI0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Brucella abortus]] | [[Category: Brucella abortus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Braden, B | + | [[Category: Braden, B C.]] |
| - | [[Category: Cauerhff, A | + | [[Category: Cauerhff, A A.]] |
| - | [[Category: Goldbaum, F | + | [[Category: Goldbaum, F A.]] |
[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
| - | [[Category: Velikovsky, C | + | [[Category: Velikovsky, C A.]] |
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:44 2008'' |
Revision as of 10:16, 21 February 2008
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CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS
Overview
We have determined the three-dimensional structure of 6, 7-dimethyl-8-ribityllumazine synthase (lumazine synthase) from Brucella abortus, the infectious organism of the disease brucellosis in animals. This enzyme catalyses the formation of 6, 7-dimethyl-8-ribityllumazine, the penultimate product in the synthesis of riboflavin. The three-dimensional X-ray crystal structure of the enzyme from B. abortus has been solved and refined at 2.7 A resolution to a final R-value of 0.18 (R(free)=0.23). The macromolecular assembly of the enzyme differs from that of the enzyme from Bacillus subtilis, the only other lumazine synthase structure known. While the protein from B. subtilis assembles into a 60 subunit icosahedral capsid built from 12 pentameric units, the enzyme from B. abortus is pentameric in its crystalline form. Nonetheless, the active sites of the two enzymes are virtually identical indicating inhibitors to theses enzymes could be effective pharmaceuticals across a broad species range. Furthermore, we compare the structures of the enzyme from B. subtilis and B. abortus and describe the C teminus structure which accounts for the differences in quaternary structure.
About this Structure
1DI0 is a Single protein structure of sequence from Brucella abortus with as ligand. Full crystallographic information is available from OCA.
Reference
Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortus., Braden BC, Velikovsky CA, Cauerhff AA, Polikarpov I, Goldbaum FA, J Mol Biol. 2000 Apr 14;297(5):1031-6. PMID:10764570
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