1dpx
From Proteopedia
(New page: 200px<br /><applet load="1dpx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dpx, resolution 1.65Å" /> '''STRUCTURE OF HEN EGG...) |
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| - | [[Image:1dpx.jpg|left|200px]]<br /><applet load="1dpx" size=" | + | [[Image:1dpx.jpg|left|200px]]<br /><applet load="1dpx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dpx, resolution 1.65Å" /> | caption="1dpx, resolution 1.65Å" /> | ||
'''STRUCTURE OF HEN EGG-WHITE LYSOZYME'''<br /> | '''STRUCTURE OF HEN EGG-WHITE LYSOZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Hen egg-white lysozyme has been crystallized at slightly alkaline pH using | + | Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it. |
==About this Structure== | ==About this Structure== | ||
| - | 1DPX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1DPX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hilgenfeld, R.]] | [[Category: Hilgenfeld, R.]] | ||
| - | [[Category: Palm, G | + | [[Category: Palm, G J.]] |
| - | [[Category: Weiss, M | + | [[Category: Weiss, M S.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: protein-chloride complex]] | [[Category: protein-chloride complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:19:10 2008'' |
Revision as of 10:19, 21 February 2008
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STRUCTURE OF HEN EGG-WHITE LYSOZYME
Overview
Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.
About this Structure
1DPX is a Single protein structure of sequence from Gallus gallus with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD., Weiss MS, Palm GJ, Hilgenfeld R, Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):952-8. PMID:10944331
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