1dt0
From Proteopedia
(New page: 200px<br /><applet load="1dt0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dt0, resolution 2.1Å" /> '''CLONING, SEQUENCE, AN...) |
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| - | [[Image:1dt0.gif|left|200px]]<br /><applet load="1dt0" size=" | + | [[Image:1dt0.gif|left|200px]]<br /><applet load="1dt0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dt0, resolution 2.1Å" /> | caption="1dt0, resolution 2.1Å" /> | ||
'''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''<br /> | '''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The gene encoding the iron-dependent superoxide dismutase from Pseudomonas | + | The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 1DT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http:// | + | 1DT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=FE:'>FE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Superoxide dismutase]] | [[Category: Superoxide dismutase]] | ||
| - | [[Category: Bond, C | + | [[Category: Bond, C J.]] |
[[Category: Flick, K.]] | [[Category: Flick, K.]] | ||
[[Category: Hajduk, R.]] | [[Category: Hajduk, R.]] | ||
[[Category: Heath, P.]] | [[Category: Heath, P.]] | ||
[[Category: Huang, J.]] | [[Category: Huang, J.]] | ||
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: FE]] | [[Category: FE]] | ||
[[Category: pseudomonas ovalis]] | [[Category: pseudomonas ovalis]] | ||
[[Category: superoxide dismutase]] | [[Category: superoxide dismutase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:09 2008'' |
Revision as of 10:20, 21 February 2008
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CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS
Overview
The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.
About this Structure
1DT0 is a Single protein structure of sequence from Pseudomonas putida with as ligand. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
Reference
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832
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