1dxe
From Proteopedia
(New page: 200px<br /><applet load="1dxe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dxe, resolution 1.8Å" /> '''2-DEHYDRO-3-DEOXY-GAL...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1dxe.gif|left|200px]]<br /><applet load="1dxe" size=" | + | [[Image:1dxe.gif|left|200px]]<br /><applet load="1dxe" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dxe, resolution 1.8Å" /> | caption="1dxe, resolution 1.8Å" /> | ||
'''2-DEHYDRO-3-DEOXY-GALACTARATE ALDOLASE FROM ESCHERICHIA COLI'''<br /> | '''2-DEHYDRO-3-DEOXY-GALACTARATE ALDOLASE FROM ESCHERICHIA COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Carbon-carbon bond formation is an essential reaction in organic chemistry | + | Carbon-carbon bond formation is an essential reaction in organic chemistry and the use of aldolase enzymes for the stereochemical control of such reactions is an attractive alternative to conventional chemical methods. Here we describe the crystal structures of a novel class II enzyme, 2-dehydro-3-deoxy-galactarate (DDG) aldolase from Escherichia coli, in the presence and absence of substrate. The crystal structure was determined by locating only four Se sites to obtain phases for 506 protein residues. The protomer displays a modified (alpha/beta)(8) barrel fold, in which the eighth alpha-helix points away from the beta-barrel instead of packing against it. Analysis of the DDG aldolase crystal structures suggests a novel aldolase mechanism in which a phosphate anion accepts the proton from the methyl group of pyruvate. |
==About this Structure== | ==About this Structure== | ||
| - | 1DXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-dehydro-3-deoxyglucarate_aldolase 2-dehydro-3-deoxyglucarate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.20 4.1.2.20] Full crystallographic information is available from [http:// | + | 1DXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-dehydro-3-deoxyglucarate_aldolase 2-dehydro-3-deoxyglucarate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.20 4.1.2.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DXE OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blackwell, N | + | [[Category: Blackwell, N C.]] |
[[Category: Izard, T.]] | [[Category: Izard, T.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 20: | Line 20: | ||
[[Category: class ii aldolase]] | [[Category: class ii aldolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:29 2008'' |
Revision as of 10:21, 21 February 2008
|
2-DEHYDRO-3-DEOXY-GALACTARATE ALDOLASE FROM ESCHERICHIA COLI
Overview
Carbon-carbon bond formation is an essential reaction in organic chemistry and the use of aldolase enzymes for the stereochemical control of such reactions is an attractive alternative to conventional chemical methods. Here we describe the crystal structures of a novel class II enzyme, 2-dehydro-3-deoxy-galactarate (DDG) aldolase from Escherichia coli, in the presence and absence of substrate. The crystal structure was determined by locating only four Se sites to obtain phases for 506 protein residues. The protomer displays a modified (alpha/beta)(8) barrel fold, in which the eighth alpha-helix points away from the beta-barrel instead of packing against it. Analysis of the DDG aldolase crystal structures suggests a novel aldolase mechanism in which a phosphate anion accepts the proton from the methyl group of pyruvate.
About this Structure
1DXE is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as 2-dehydro-3-deoxyglucarate aldolase, with EC number 4.1.2.20 Full crystallographic information is available from OCA.
Reference
Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism., Izard T, Blackwell NC, EMBO J. 2000 Aug 1;19(15):3849-56. PMID:10921867
Page seeded by OCA on Thu Feb 21 12:21:29 2008
