1ebu
From Proteopedia
(New page: 200px<br /><applet load="1ebu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebu, resolution 2.60Å" /> '''HOMOSERINE DEHYDROGE...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ebu.jpg|left|200px]]<br /><applet load="1ebu" size=" | + | [[Image:1ebu.jpg|left|200px]]<br /><applet load="1ebu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ebu, resolution 2.60Å" /> | caption="1ebu, resolution 2.60Å" /> | ||
'''HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE'''<br /> | '''HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the antifungal drug target homoserine dehydrogenase (HSD) | + | The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry. |
==About this Structure== | ==About this Structure== | ||
| - | 1EBU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NA, NDA and HSE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http:// | + | 1EBU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=NDA:'>NDA</scene> and <scene name='pdbligand=HSE:'>HSE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBU OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berghuis, A | + | [[Category: Berghuis, A M.]] |
[[Category: DeLaBarre, B.]] | [[Category: DeLaBarre, B.]] | ||
| - | [[Category: Thompson, P | + | [[Category: Thompson, P R.]] |
| - | [[Category: Wright, G | + | [[Category: Wright, G D.]] |
[[Category: HSE]] | [[Category: HSE]] | ||
[[Category: NA]] | [[Category: NA]] | ||
| Line 27: | Line 27: | ||
[[Category: ternary]] | [[Category: ternary]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:07 2008'' |
Revision as of 10:26, 21 February 2008
|
HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE
Overview
The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
About this Structure
1EBU is a Single protein structure of sequence from Saccharomyces cerevisiae with , and as ligands. Active as Homoserine dehydrogenase, with EC number 1.1.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284
Page seeded by OCA on Thu Feb 21 12:26:07 2008
