This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ek0
From Proteopedia
(New page: 200px<br /><applet load="1ek0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ek0, resolution 1.48Å" /> '''GPPNHP-BOUND YPT51 A...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ek0.jpg|left|200px]]<br /><applet load="1ek0" size=" | + | [[Image:1ek0.jpg|left|200px]]<br /><applet load="1ek0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ek0, resolution 1.48Å" /> | caption="1ek0, resolution 1.48Å" /> | ||
'''GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION'''<br /> | '''GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ypt/Rab proteins are membrane-associated small GTP-binding proteins which | + | Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 A high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate. |
==About this Structure== | ==About this Structure== | ||
| - | 1EK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG, NI, GNP and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1EK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=GNP:'>GNP</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EK0 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Esters, H.]] | [[Category: Esters, H.]] | ||
| - | [[Category: Scheidig, A | + | [[Category: Scheidig, A J.]] |
[[Category: GDP]] | [[Category: GDP]] | ||
[[Category: GNP]] | [[Category: GNP]] | ||
| Line 26: | Line 26: | ||
[[Category: ypt/rab protein]] | [[Category: ypt/rab protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:34 2008'' |
Revision as of 10:28, 21 February 2008
|
GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION
Overview
Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 A high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate.
About this Structure
1EK0 is a Single protein structure of sequence from Saccharomyces cerevisiae with , , and as ligands. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis., Esters H, Alexandrov K, Constantinescu AT, Goody RS, Scheidig AJ, J Mol Biol. 2000 Apr 21;298(1):111-21. PMID:10756108
Page seeded by OCA on Thu Feb 21 12:28:34 2008
Categories: Saccharomyces cerevisiae | Single protein | Esters, H. | Scheidig, A J. | GDP | GNP | MG | NI | Endocytosis | G protein | Gtp hydrolysis | Hydrolase | Vesicular traffic | Ypt/rab protein
