1ek0
From Proteopedia
(New page: 200px<br /><applet load="1ek0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ek0, resolution 1.48Å" /> '''GPPNHP-BOUND YPT51 A...) |
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| - | [[Image:1ek0.jpg|left|200px]]<br /><applet load="1ek0" size=" | + | [[Image:1ek0.jpg|left|200px]]<br /><applet load="1ek0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ek0, resolution 1.48Å" /> | caption="1ek0, resolution 1.48Å" /> | ||
'''GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION'''<br /> | '''GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ypt/Rab proteins are membrane-associated small GTP-binding proteins which | + | Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 A high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate. |
==About this Structure== | ==About this Structure== | ||
| - | 1EK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG, NI, GNP and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1EK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=GNP:'>GNP</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EK0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Esters, H.]] | [[Category: Esters, H.]] | ||
| - | [[Category: Scheidig, A | + | [[Category: Scheidig, A J.]] |
[[Category: GDP]] | [[Category: GDP]] | ||
[[Category: GNP]] | [[Category: GNP]] | ||
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[[Category: ypt/rab protein]] | [[Category: ypt/rab protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:34 2008'' |
Revision as of 10:28, 21 February 2008
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GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION
Overview
Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 A high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate.
About this Structure
1EK0 is a Single protein structure of sequence from Saccharomyces cerevisiae with , , and as ligands. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis., Esters H, Alexandrov K, Constantinescu AT, Goody RS, Scheidig AJ, J Mol Biol. 2000 Apr 21;298(1):111-21. PMID:10756108
Page seeded by OCA on Thu Feb 21 12:28:34 2008
Categories: Saccharomyces cerevisiae | Single protein | Esters, H. | Scheidig, A J. | GDP | GNP | MG | NI | Endocytosis | G protein | Gtp hydrolysis | Hydrolase | Vesicular traffic | Ypt/rab protein
