1ekz
From Proteopedia
(New page: 200px<br /><applet load="1ekz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ekz" /> '''NMR STRUCTURE OF THE COMPLEX BETWEEN THE THI...) |
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| - | [[Image:1ekz.jpg|left|200px]]<br /><applet load="1ekz" size=" | + | [[Image:1ekz.jpg|left|200px]]<br /><applet load="1ekz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ekz" /> | caption="1ekz" /> | ||
'''NMR STRUCTURE OF THE COMPLEX BETWEEN THE THIRD DSRBD FROM DROSOPHILA STAUFEN AND A RNA HAIRPIN'''<br /> | '''NMR STRUCTURE OF THE COMPLEX BETWEEN THE THIRD DSRBD FROM DROSOPHILA STAUFEN AND A RNA HAIRPIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The double-stranded RNA-binding domain (dsRBD) is a common RNA-binding | + | The double-stranded RNA-binding domain (dsRBD) is a common RNA-binding motif found in many proteins involved in RNA maturation and localization. To determine how this domain recognizes RNA, we have studied the third dsRBD from Drosophila Staufen. The domain binds optimally to RNA stem-loops containing 12 uninterrupted base pairs, and we have identified the amino acids required for this interaction. By mutating these residues in a staufen transgene, we show that the RNA-binding activity of dsRBD3 is required in vivo for Staufen-dependent localization of bicoid and oskar mRNAs. Using high-resolution NMR, we have determined the structure of the complex between dsRBD3 and an RNA stem-loop. The dsRBD recognizes the shape of A-form dsRNA through interactions between conserved residues within loop 2 and the minor groove, and between loop 4 and the phosphodiester backbone across the adjacent major groove. In addition, helix alpha1 interacts with the single-stranded loop that caps the RNA helix. Interactions between helix alpha1 and single-stranded RNA may be important determinants of the specificity of dsRBD proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1EKZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1EKZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EKZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bycroft, M.]] | [[Category: Bycroft, M.]] | ||
[[Category: Grunert, S.]] | [[Category: Grunert, S.]] | ||
| - | [[Category: Johnston, D | + | [[Category: Johnston, D St.]] |
[[Category: Ramos, A.]] | [[Category: Ramos, A.]] | ||
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
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[[Category: rna hairpin]] | [[Category: rna hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:52 2008'' |
Revision as of 10:28, 21 February 2008
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NMR STRUCTURE OF THE COMPLEX BETWEEN THE THIRD DSRBD FROM DROSOPHILA STAUFEN AND A RNA HAIRPIN
Overview
The double-stranded RNA-binding domain (dsRBD) is a common RNA-binding motif found in many proteins involved in RNA maturation and localization. To determine how this domain recognizes RNA, we have studied the third dsRBD from Drosophila Staufen. The domain binds optimally to RNA stem-loops containing 12 uninterrupted base pairs, and we have identified the amino acids required for this interaction. By mutating these residues in a staufen transgene, we show that the RNA-binding activity of dsRBD3 is required in vivo for Staufen-dependent localization of bicoid and oskar mRNAs. Using high-resolution NMR, we have determined the structure of the complex between dsRBD3 and an RNA stem-loop. The dsRBD recognizes the shape of A-form dsRNA through interactions between conserved residues within loop 2 and the minor groove, and between loop 4 and the phosphodiester backbone across the adjacent major groove. In addition, helix alpha1 interacts with the single-stranded loop that caps the RNA helix. Interactions between helix alpha1 and single-stranded RNA may be important determinants of the specificity of dsRBD proteins.
About this Structure
1EKZ is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
RNA recognition by a Staufen double-stranded RNA-binding domain., Ramos A, Grunert S, Adams J, Micklem DR, Proctor MR, Freund S, Bycroft M, St Johnston D, Varani G, EMBO J. 2000 Mar 1;19(5):997-1009. PMID:10698941
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