1eo6
From Proteopedia
(New page: 200px<br /><applet load="1eo6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eo6, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1eo6.gif|left|200px]]<br /><applet load="1eo6" size=" | + | [[Image:1eo6.gif|left|200px]]<br /><applet load="1eo6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1eo6, resolution 1.8Å" /> | caption="1eo6, resolution 1.8Å" /> | ||
'''CRYSTAL STRUCTURE OF GATE-16'''<br /> | '''CRYSTAL STRUCTURE OF GATE-16'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The GATE-16 protein participates in intra-Golgi transport and can | + | The GATE-16 protein participates in intra-Golgi transport and can associate with the N-ethylmaleimide-sensitive fusion protein and with Golgi SNAREs. The yeast ortholog of GATE-16 is the autophagocytosis factor Aut7p. GATE-16 is also closely related to the GABA receptor-associated protein (GABARAP), which has been proposed to cluster neurotransmitter receptors by mediating interaction with the cytoskeleton, and to the light chain-3 subunit of the neuronal microtubule-associated protein complex. Here, we present the crystal structure of GATE-16 refined to 1.8 A resolution. GATE-16 contains a ubiquitin fold decorated by two additional N-terminal helices. Proteins with strong structural similarity but no detectable sequence homology to GATE-16 include Ras effectors that mediate diverse downstream functions, but each interacts with Ras by forming pseudo-continuous beta-sheets. The GATE-16 surface suggests that it binds its targets in a similar manner. Moreover, a second potential protein-protein interaction site on GATE-16 may explain the adapter activity observed for members of the GATE-16 family. |
==About this Structure== | ==About this Structure== | ||
| - | 1EO6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | + | 1EO6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EO6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ubiquitin fold]] | [[Category: ubiquitin fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:48 2008'' |
Revision as of 10:29, 21 February 2008
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CRYSTAL STRUCTURE OF GATE-16
Overview
The GATE-16 protein participates in intra-Golgi transport and can associate with the N-ethylmaleimide-sensitive fusion protein and with Golgi SNAREs. The yeast ortholog of GATE-16 is the autophagocytosis factor Aut7p. GATE-16 is also closely related to the GABA receptor-associated protein (GABARAP), which has been proposed to cluster neurotransmitter receptors by mediating interaction with the cytoskeleton, and to the light chain-3 subunit of the neuronal microtubule-associated protein complex. Here, we present the crystal structure of GATE-16 refined to 1.8 A resolution. GATE-16 contains a ubiquitin fold decorated by two additional N-terminal helices. Proteins with strong structural similarity but no detectable sequence homology to GATE-16 include Ras effectors that mediate diverse downstream functions, but each interacts with Ras by forming pseudo-continuous beta-sheets. The GATE-16 surface suggests that it binds its targets in a similar manner. Moreover, a second potential protein-protein interaction site on GATE-16 may explain the adapter activity observed for members of the GATE-16 family.
About this Structure
1EO6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p., Paz Y, Elazar Z, Fass D, J Biol Chem. 2000 Aug 18;275(33):25445-50. PMID:10856287
Page seeded by OCA on Thu Feb 21 12:29:48 2008
