1ev0
From Proteopedia
(New page: 200px<br /><applet load="1ev0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ev0" /> '''SOLUTION STRUCTURE OF THE MINE TOPOLOGICAL S...) |
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| - | [[Image:1ev0.jpg|left|200px]]<br /><applet load="1ev0" size=" | + | [[Image:1ev0.jpg|left|200px]]<br /><applet load="1ev0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ev0" /> | caption="1ev0" /> | ||
'''SOLUTION STRUCTURE OF THE MINE TOPOLOGICAL SPECIFICITY DOMAIN'''<br /> | '''SOLUTION STRUCTURE OF THE MINE TOPOLOGICAL SPECIFICITY DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Correct positioning of the division septum in Escherichia coli depends on | + | Correct positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins. Topological specificity is conferred on the MinCD division inhibitor by MinE, which counters MinCD activity only in the vicinity of the preferred midcell division site. Here we report the structure of the homodimeric topological specificity domain of Escherichia coli MinE and show that it forms a novel alphabeta sandwich. Structure-directed mutagenesis of conserved surface residues has enabled us to identify a spatially restricted site on the surface of the protein that is critical for the topological specificity function of MinE. |
==About this Structure== | ==About this Structure== | ||
| - | 1EV0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1EV0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: King, G | + | [[Category: King, G F.]] |
| - | [[Category: Maciejewski, M | + | [[Category: Maciejewski, M W.]] |
| - | [[Category: Mullen, G | + | [[Category: Mullen, G P.]] |
[[Category: Pan, B.]] | [[Category: Pan, B.]] | ||
[[Category: cell division]] | [[Category: cell division]] | ||
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[[Category: topological specificity]] | [[Category: topological specificity]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:47 2008'' |
Revision as of 10:31, 21 February 2008
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SOLUTION STRUCTURE OF THE MINE TOPOLOGICAL SPECIFICITY DOMAIN
Overview
Correct positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins. Topological specificity is conferred on the MinCD division inhibitor by MinE, which counters MinCD activity only in the vicinity of the preferred midcell division site. Here we report the structure of the homodimeric topological specificity domain of Escherichia coli MinE and show that it forms a novel alphabeta sandwich. Structure-directed mutagenesis of conserved surface residues has enabled us to identify a spatially restricted site on the surface of the protein that is critical for the topological specificity function of MinE.
About this Structure
1EV0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis for the topological specificity function of MinE., King GF, Shih YL, Maciejewski MW, Bains NP, Pan B, Rowland SL, Mullen GP, Rothfield LI, Nat Struct Biol. 2000 Nov;7(11):1013-7. PMID:11062554
Page seeded by OCA on Thu Feb 21 12:31:47 2008
