1f26
From Proteopedia
(New page: 200px<br /><applet load="1f26" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f26, resolution 1.4Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1f26.gif|left|200px]]<br /><applet load="1f26" size=" | + | [[Image:1f26.gif|left|200px]]<br /><applet load="1f26" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f26, resolution 1.4Å" /> | caption="1f26, resolution 1.4Å" /> | ||
'''CRYSTAL STRUCTURE OF NO COMPLEX OF THR243VAL MUTANTS OF CYTOCHROME P450NOR'''<br /> | '''CRYSTAL STRUCTURE OF NO COMPLEX OF THR243VAL MUTANTS OF CYTOCHROME P450NOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Threonine 243 of cytochrome P450nor (fungal nitric oxide reductase) | + | Threonine 243 of cytochrome P450nor (fungal nitric oxide reductase) corresponds to the 'conserved' Thr in the long I helix of monooxygenase cytochrome P450s. In P450nor, the replacement of Thr243 with Asn, Ala or Val makes the enzymatic activity dramatically reduce. In order to understand the roles of Thr243 in the reduction reaction of NO by P450nor, the crystal structures of three Thr243 mutants (Thr243-->Asn, Thr243-->Val, Thr243-->Ala) of P450nor were determined at a 1.4-A resolution and at cryogenic temperature. However, the hydrogen-bonding pattern in the heme pocket of these mutants is essentially similar for that of the WT enzyme. This suggests that the determination of the structure of the NADH complex of P450nor is required, in order to evaluate the role of Thr243 in its enzymatic reaction. We attempted to crystallize the NADH complex under several conditions, but have not yet been successful. |
==About this Structure== | ==About this Structure== | ||
| - | 1F26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum] with HEM, NO and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_reductase Nitric-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.7 1.7.99.7] Full crystallographic information is available from [http:// | + | 1F26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum] with <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=NO:'>NO</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_reductase Nitric-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.7 1.7.99.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F26 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nitric-oxide reductase]] | [[Category: Nitric-oxide reductase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Park, S | + | [[Category: Park, S Y.]] |
[[Category: Shimizu, H.]] | [[Category: Shimizu, H.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: nitric oxide reductase]] | [[Category: nitric oxide reductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:01 2008'' |
Revision as of 10:34, 21 February 2008
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CRYSTAL STRUCTURE OF NO COMPLEX OF THR243VAL MUTANTS OF CYTOCHROME P450NOR
Overview
Threonine 243 of cytochrome P450nor (fungal nitric oxide reductase) corresponds to the 'conserved' Thr in the long I helix of monooxygenase cytochrome P450s. In P450nor, the replacement of Thr243 with Asn, Ala or Val makes the enzymatic activity dramatically reduce. In order to understand the roles of Thr243 in the reduction reaction of NO by P450nor, the crystal structures of three Thr243 mutants (Thr243-->Asn, Thr243-->Val, Thr243-->Ala) of P450nor were determined at a 1.4-A resolution and at cryogenic temperature. However, the hydrogen-bonding pattern in the heme pocket of these mutants is essentially similar for that of the WT enzyme. This suggests that the determination of the structure of the NADH complex of P450nor is required, in order to evaluate the role of Thr243 in its enzymatic reaction. We attempted to crystallize the NADH complex under several conditions, but have not yet been successful.
About this Structure
1F26 is a Single protein structure of sequence from Fusarium oxysporum with , and as ligands. Active as Nitric-oxide reductase, with EC number 1.7.99.7 Full crystallographic information is available from OCA.
Reference
Mutation effects of a conserved threonine (Thr243) of cytochrome P450nor on its structure and function., Obayashi E, Shimizu H, Park SY, Shoun H, Shiro Y, J Inorg Biochem. 2000 Nov;82(1-4):103-11. PMID:11132616
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