1f99
From Proteopedia
(New page: 200px<br /><applet load="1f99" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f99, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1f99.gif|left|200px]]<br /><applet load="1f99" size=" | + | [[Image:1f99.gif|left|200px]]<br /><applet load="1f99" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f99, resolution 2.4Å" /> | caption="1f99, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of R-phycocyanin from Polysiphonia urceolata | + | The crystal structure of R-phycocyanin from Polysiphonia urceolata (R-PC-PU) at 2.4 A is reported. The R-PC-PU crystal belongs to space group P4(3)2(1)2 with cell parameters a = 135.1 A, c = 210.0 A, and alpha = beta = gamma = 90 degrees. The structure was determined by molecular replacement. The crystallographic R-factor of the refined model is 0.189 (R(free) = 0.239). Comparison of the microenvironment of chromophore beta 155 in R-PC-PU and in C-PC from Fremyolla diphosiphon (C-PC-FD) reveals that their spectral differences may be caused by their different alpha 28 residues. In the R-PC-PU crystal structure, two (alpha beta)(3) trimers assemble face to face to form a hexamer, and two such hexamers assemble in two novel side-to-side arrangements. Possible models for the energy transfer from phycoerythrin to phycocyanin and from phycocyanin to allophycocyanin are proposed based on several phycobiliprotein crystal structures. |
==About this Structure== | ==About this Structure== | ||
| - | 1F99 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata] with BLA, CYC and PEB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1F99 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Polysiphonia_urceolata Polysiphonia urceolata] with <scene name='pdbligand=BLA:'>BLA</scene>, <scene name='pdbligand=CYC:'>CYC</scene> and <scene name='pdbligand=PEB:'>PEB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F99 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Polysiphonia urceolata]] | [[Category: Polysiphonia urceolata]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chang, W | + | [[Category: Chang, W R.]] |
[[Category: Jiang, T.]] | [[Category: Jiang, T.]] | ||
| - | [[Category: Liang, D | + | [[Category: Liang, D C.]] |
[[Category: BLA]] | [[Category: BLA]] | ||
[[Category: CYC]] | [[Category: CYC]] | ||
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[[Category: r-phycocyanin]] | [[Category: r-phycocyanin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:36:16 2008'' |
Revision as of 10:36, 21 February 2008
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CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION
Overview
The crystal structure of R-phycocyanin from Polysiphonia urceolata (R-PC-PU) at 2.4 A is reported. The R-PC-PU crystal belongs to space group P4(3)2(1)2 with cell parameters a = 135.1 A, c = 210.0 A, and alpha = beta = gamma = 90 degrees. The structure was determined by molecular replacement. The crystallographic R-factor of the refined model is 0.189 (R(free) = 0.239). Comparison of the microenvironment of chromophore beta 155 in R-PC-PU and in C-PC from Fremyolla diphosiphon (C-PC-FD) reveals that their spectral differences may be caused by their different alpha 28 residues. In the R-PC-PU crystal structure, two (alpha beta)(3) trimers assemble face to face to form a hexamer, and two such hexamers assemble in two novel side-to-side arrangements. Possible models for the energy transfer from phycoerythrin to phycocyanin and from phycocyanin to allophycocyanin are proposed based on several phycobiliprotein crystal structures.
About this Structure
1F99 is a Protein complex structure of sequences from Polysiphonia urceolata with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of R-phycocyanin and possible energy transfer pathways in the phycobilisome., Jiang T, Zhang JP, Chang WR, Liang DC, Biophys J. 2001 Aug;81(2):1171-9. PMID:11463658
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