1fca
From Proteopedia
(New page: 200px<br /><applet load="1fca" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fca, resolution 1.8Å" /> '''STRUCTURE OF THE FERR...) |
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| - | [[Image:1fca.jpg|left|200px]]<br /><applet load="1fca" size=" | + | [[Image:1fca.jpg|left|200px]]<br /><applet load="1fca" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fca, resolution 1.8Å" /> | caption="1fca, resolution 1.8Å" /> | ||
'''STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION'''<br /> | '''STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ferredoxins (Fd) are electron-carrier proteins, the active sites of which | + | Ferredoxins (Fd) are electron-carrier proteins, the active sites of which are organized around clusters made of iron and inorganic sulfur. The Fd from Clostridium acidurici is 55 amino acids long and contains two [4Fe-4S] clusters. Crystals have been obtained in the space group P4(3)2(1)2, a = b = 34.441 (5), c = 74.778 (9) A. The structure was solved by molecular replacement using the Fd from Peptostreptcoccus asaccharolyticus as a search model, these two ferredoxins having 37 residues in common. Refinement using molecular-dynamics techniques was then initiated. Successive rounds of model building and refinement gave a structure that includes 45 water molecules with R = 15%. At this stage, the electron-density map clearly revealed discrepancies in the position of two amino acids in the published primary sequence. Refinement based on these modifications led to R = 14.3% for 3921 reflections up to 1.8 A, resolution. The geometry of the two clusters has been found to be in good agreement with that previously obtained at a lower resolution. Interactions of polypeptide chain with the [4Fe-4S] clusters, the cluster geometry as well as the hydrogen bonds involving S, Sgamma, N and water molecules are reported. |
==About this Structure== | ==About this Structure== | ||
| - | 1FCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_acidurici Clostridium acidurici] with SF4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_acidurici Clostridium acidurici] with <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Clostridium acidurici]] | [[Category: Clostridium acidurici]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jesior, J | + | [[Category: Jesior, J C.]] |
[[Category: Tranqui, D.]] | [[Category: Tranqui, D.]] | ||
[[Category: SF4]] | [[Category: SF4]] | ||
[[Category: electron transport (cytochrome)]] | [[Category: electron transport (cytochrome)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:12 2008'' |
Revision as of 10:37, 21 February 2008
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STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION
Overview
Ferredoxins (Fd) are electron-carrier proteins, the active sites of which are organized around clusters made of iron and inorganic sulfur. The Fd from Clostridium acidurici is 55 amino acids long and contains two [4Fe-4S] clusters. Crystals have been obtained in the space group P4(3)2(1)2, a = b = 34.441 (5), c = 74.778 (9) A. The structure was solved by molecular replacement using the Fd from Peptostreptcoccus asaccharolyticus as a search model, these two ferredoxins having 37 residues in common. Refinement using molecular-dynamics techniques was then initiated. Successive rounds of model building and refinement gave a structure that includes 45 water molecules with R = 15%. At this stage, the electron-density map clearly revealed discrepancies in the position of two amino acids in the published primary sequence. Refinement based on these modifications led to R = 14.3% for 3921 reflections up to 1.8 A, resolution. The geometry of the two clusters has been found to be in good agreement with that previously obtained at a lower resolution. Interactions of polypeptide chain with the [4Fe-4S] clusters, the cluster geometry as well as the hydrogen bonds involving S, Sgamma, N and water molecules are reported.
About this Structure
1FCA is a Single protein structure of sequence from Clostridium acidurici with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:15299316
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