1fcs
From Proteopedia
(New page: 200px<br /><applet load="1fcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fcs, resolution 1.6Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1fcs.gif|left|200px]]<br /><applet load="1fcs" size=" | + | [[Image:1fcs.gif|left|200px]]<br /><applet load="1fcs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fcs, resolution 1.6Å" /> | caption="1fcs, resolution 1.6Å" /> | ||
'''CRYSTAL STRUCTURE OF A DISTAL SITE DOUBLE MUTANT OF SPERM WHALE MYOGLOBIN AT 1.6 ANGSTROMS RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF A DISTAL SITE DOUBLE MUTANT OF SPERM WHALE MYOGLOBIN AT 1.6 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of sperm whale myoglobin | + | The three-dimensional structure of sperm whale myoglobin His64(E7)-->Val,Thr67(E10)-->Arg double mutant has been studied by X-ray crystallography at 1.6 A resolution, and refined to a crystallographic R-factor of 0.197. The Arg67(E10) side chain is extended in the direction of the ligand binding site, and its NH1 atom is at a distance of 3.11 A from the NH1 atom of Arg45(CD3), which is also pointing towards the distal site. Both are kept in this position by hydrogen bonding and electrostatic interactions with a solvent sulfate ion, located amongst the two, on the protein surface. No liganded water molecule is present at the sixth coordination position of the Fe(III) heme. |
==About this Structure== | ==About this Structure== | ||
| - | 1FCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with SO4 and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1FCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Physeter catodon]] | [[Category: Physeter catodon]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Allocatelli, C | + | [[Category: Allocatelli, C Travaglini.]] |
[[Category: Bolognesi, M.]] | [[Category: Bolognesi, M.]] | ||
[[Category: Brancaccio, A.]] | [[Category: Brancaccio, A.]] | ||
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[[Category: oxygen transport]] | [[Category: oxygen transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:20 2008'' |
Revision as of 10:37, 21 February 2008
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CRYSTAL STRUCTURE OF A DISTAL SITE DOUBLE MUTANT OF SPERM WHALE MYOGLOBIN AT 1.6 ANGSTROMS RESOLUTION
Overview
The three-dimensional structure of sperm whale myoglobin His64(E7)-->Val,Thr67(E10)-->Arg double mutant has been studied by X-ray crystallography at 1.6 A resolution, and refined to a crystallographic R-factor of 0.197. The Arg67(E10) side chain is extended in the direction of the ligand binding site, and its NH1 atom is at a distance of 3.11 A from the NH1 atom of Arg45(CD3), which is also pointing towards the distal site. Both are kept in this position by hydrogen bonding and electrostatic interactions with a solvent sulfate ion, located amongst the two, on the protein surface. No liganded water molecule is present at the sixth coordination position of the Fe(III) heme.
About this Structure
1FCS is a Single protein structure of sequence from Physeter catodon with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 A resolution., Rizzi M, Bolognesi M, Coda A, Cutruzzola F, Allocatelli CT, Brancaccio A, Brunori M, FEBS Lett. 1993 Mar 29;320(1):13-6. PMID:8462669
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