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1fer
From Proteopedia
(New page: 200px<br /><applet load="1fer" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fer, resolution 2.3Å" /> '''STRUCTURE AT PH 6.5 O...) |
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| - | [[Image:1fer.jpg|left|200px]]<br /><applet load="1fer" size=" | + | [[Image:1fer.jpg|left|200px]]<br /><applet load="1fer" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fer, resolution 2.3Å" /> | caption="1fer, resolution 2.3Å" /> | ||
'''STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION'''<br /> | '''STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein | + | Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein composed of 106 amino acids, seven Fe atoms and eight inorganic S* atoms. A crystallographic redetermination of its structure showed the originally reported structure to be incorrect. We report here the crystal structure of AvFdI at pH 6.5. Extensive refinement has led to a final R value of 0.170 for all 6986 non-extinct reflections in the range 10-2.3 A using a solvent model which includes 98 discrete solvent atoms with occupancies between 0.3 and 1.0 and an average B value of 22.5 A(2). The first half of the peptide chain closely resembles that of the 55-residue ferredoxin from Peptococcus aerogenes (PaFd), while the remainder consists of three turns of helix and a series of loops which form a cap over part of the molecular core. Despite the similarities in structure and surroundings, the corresponding 4Fe4S* clusters in PaFd and AvFdI have strikingly different redox potentials; a possible explanation has been sought in the differing hydration models for the two molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1FER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with F3S and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1FER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=F3S:'>F3S</scene> and <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FER OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Azotobacter vinelandii]] | [[Category: Azotobacter vinelandii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jensen, L | + | [[Category: Jensen, L H.]] |
| - | [[Category: Merritt, E | + | [[Category: Merritt, E A.]] |
| - | [[Category: Orme-Johnson, W | + | [[Category: Orme-Johnson, W H.]] |
| - | [[Category: Sieker, L | + | [[Category: Sieker, L C.]] |
| - | [[Category: Stout, G | + | [[Category: Stout, G H.]] |
[[Category: Turley, S.]] | [[Category: Turley, S.]] | ||
[[Category: F3S]] | [[Category: F3S]] | ||
| Line 23: | Line 23: | ||
[[Category: electron transfer(iron-sulfur protein)]] | [[Category: electron transfer(iron-sulfur protein)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:54 2008'' |
Revision as of 10:37, 21 February 2008
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STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION
Overview
Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein composed of 106 amino acids, seven Fe atoms and eight inorganic S* atoms. A crystallographic redetermination of its structure showed the originally reported structure to be incorrect. We report here the crystal structure of AvFdI at pH 6.5. Extensive refinement has led to a final R value of 0.170 for all 6986 non-extinct reflections in the range 10-2.3 A using a solvent model which includes 98 discrete solvent atoms with occupancies between 0.3 and 1.0 and an average B value of 22.5 A(2). The first half of the peptide chain closely resembles that of the 55-residue ferredoxin from Peptococcus aerogenes (PaFd), while the remainder consists of three turns of helix and a series of loops which form a cap over part of the molecular core. Despite the similarities in structure and surroundings, the corresponding 4Fe4S* clusters in PaFd and AvFdI have strikingly different redox potentials; a possible explanation has been sought in the differing hydration models for the two molecules.
About this Structure
1FER is a Single protein structure of sequence from Azotobacter vinelandii with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3 A resolution., Merritt EA, Stout GH, Turley S, Sieker LC, Jehsen LH, Orme-Johnson WH, Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):272-81. PMID:15299532
Page seeded by OCA on Thu Feb 21 12:37:54 2008
