1fwx
From Proteopedia
(New page: 200px<br /><applet load="1fwx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fwx, resolution 1.60Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1fwx.jpg|left|200px]]<br /><applet load="1fwx" size=" | + | [[Image:1fwx.jpg|left|200px]]<br /><applet load="1fwx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fwx, resolution 1.60Å" /> | caption="1fwx, resolution 1.60Å" /> | ||
'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS'''<br /> | '''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of | + | Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of N(2)O to N(2). The crystal structure of N2ORs from Pseudomonas nautica (Pn) and Paracoccus denitrificans (Pd) were solved at resolutions of 2.4 and 1.6 A, respectively. The Pn N2OR structure revealed that the catalytic CuZ center belongs to a new type of metal cluster in which four copper ions are liganded by seven histidine residues. A bridging oxygen moiety and two other hydroxide ligands were proposed to complete the ligation scheme (Brown, K., Tegoni, M., Prudencio, M., Pereira, A. S., Besson, S., Moura, J. J. G., Moura, I., and Cambillau, C. (2000) Nat. Struct. Biol. 7, 191-195). However, in the CuZ cluster, inorganic sulfur chemical determination and the high resolution structure of Pd N2OR identified a bridging inorganic sulfur instead of an oxygen. This result reconciles the novel CuZ cluster with the hitherto puzzling spectroscopic data. |
==About this Structure== | ==About this Structure== | ||
| - | 1FWX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with CL, CA, CUA and CUZ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6] Full crystallographic information is available from [http:// | + | 1FWX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=CUA:'>CUA</scene> and <scene name='pdbligand=CUZ:'>CUZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Haltia, T.]] | [[Category: Haltia, T.]] | ||
[[Category: Moura, I.]] | [[Category: Moura, I.]] | ||
| - | [[Category: Moura, J | + | [[Category: Moura, J J.]] |
[[Category: Saraste, M.]] | [[Category: Saraste, M.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
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[[Category: cuz site]] | [[Category: cuz site]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:33 2008'' |
Revision as of 10:43, 21 February 2008
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CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS
Overview
Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of N(2)O to N(2). The crystal structure of N2ORs from Pseudomonas nautica (Pn) and Paracoccus denitrificans (Pd) were solved at resolutions of 2.4 and 1.6 A, respectively. The Pn N2OR structure revealed that the catalytic CuZ center belongs to a new type of metal cluster in which four copper ions are liganded by seven histidine residues. A bridging oxygen moiety and two other hydroxide ligands were proposed to complete the ligation scheme (Brown, K., Tegoni, M., Prudencio, M., Pereira, A. S., Besson, S., Moura, J. J. G., Moura, I., and Cambillau, C. (2000) Nat. Struct. Biol. 7, 191-195). However, in the CuZ cluster, inorganic sulfur chemical determination and the high resolution structure of Pd N2OR identified a bridging inorganic sulfur instead of an oxygen. This result reconciles the novel CuZ cluster with the hitherto puzzling spectroscopic data.
About this Structure
1FWX is a Single protein structure of sequence from Paracoccus denitrificans with , , and as ligands. Active as Nitrous-oxide reductase, with EC number 1.7.99.6 Full crystallographic information is available from OCA.
Reference
Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase. Evidence of a bridging inorganic sulfur., Brown K, Djinovic-Carugo K, Haltia T, Cabrito I, Saraste M, Moura JJ, Moura I, Tegoni M, Cambillau C, J Biol Chem. 2000 Dec 29;275(52):41133-6. PMID:11024061
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