1g4y
From Proteopedia
(New page: 200px<br /><applet load="1g4y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g4y, resolution 1.60Å" /> '''1.60 A CRYSTAL STRUC...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1g4y.jpg|left|200px]]<br /><applet load="1g4y" size=" | + | [[Image:1g4y.jpg|left|200px]]<br /><applet load="1g4y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g4y, resolution 1.60Å" /> | caption="1g4y, resolution 1.60Å" /> | ||
'''1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN'''<br /> | '''1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Small-conductance Ca2+-activated K+ channels (SK channels) are independent | + | Small-conductance Ca2+-activated K+ channels (SK channels) are independent of voltage and gated solely by intracellular Ca2+. These membrane channels are heteromeric complexes that comprise pore-forming alpha-subunits and the Ca2+-binding protein calmodulin (CaM). CaM binds to the SK channel through the CaM-binding domain (CaMBD), which is located in an intracellular region of the alpha-subunit immediately carboxy-terminal to the pore. Channel opening is triggered when Ca2+ binds the EF hands in the N-lobe of CaM. Here we report the 1.60 A crystal structure of the SK channel CaMBD/Ca2+/CaM complex. The CaMBD forms an elongated dimer with a CaM molecule bound at each end; each CaM wraps around three alpha-helices, two from one CaMBD subunit and one from the other. As only the CaM N-lobe has bound Ca2+, the structure provides a view of both calcium-dependent and -independent CaM/protein interactions. Together with biochemical data, the structure suggests a possible gating mechanism for the SK channel. |
==About this Structure== | ==About this Structure== | ||
| - | 1G4Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1G4Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4Y OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Adelman, J | + | [[Category: Adelman, J P.]] |
| - | [[Category: Bachinger, H | + | [[Category: Bachinger, H P.]] |
[[Category: Rivard, A.]] | [[Category: Rivard, A.]] | ||
| - | [[Category: Schumacher, M | + | [[Category: Schumacher, M A.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| Line 24: | Line 24: | ||
[[Category: small-conductance calcium-activated potassium channel]] | [[Category: small-conductance calcium-activated potassium channel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:07 2008'' |
Revision as of 10:46, 21 February 2008
|
1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN
Overview
Small-conductance Ca2+-activated K+ channels (SK channels) are independent of voltage and gated solely by intracellular Ca2+. These membrane channels are heteromeric complexes that comprise pore-forming alpha-subunits and the Ca2+-binding protein calmodulin (CaM). CaM binds to the SK channel through the CaM-binding domain (CaMBD), which is located in an intracellular region of the alpha-subunit immediately carboxy-terminal to the pore. Channel opening is triggered when Ca2+ binds the EF hands in the N-lobe of CaM. Here we report the 1.60 A crystal structure of the SK channel CaMBD/Ca2+/CaM complex. The CaMBD forms an elongated dimer with a CaM molecule bound at each end; each CaM wraps around three alpha-helices, two from one CaMBD subunit and one from the other. As only the CaM N-lobe has bound Ca2+, the structure provides a view of both calcium-dependent and -independent CaM/protein interactions. Together with biochemical data, the structure suggests a possible gating mechanism for the SK channel.
About this Structure
1G4Y is a Protein complex structure of sequences from Rattus norvegicus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin., Schumacher MA, Rivard AF, Bachinger HP, Adelman JP, Nature. 2001 Apr 26;410(6832):1120-4. PMID:11323678
Page seeded by OCA on Thu Feb 21 12:46:07 2008
