1g7p

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(New page: 200px<br /><applet load="1g7p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g7p, resolution 1.50&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1g7p.gif|left|200px]]<br /><applet load="1g7p" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1g7p.gif|left|200px]]<br /><applet load="1g7p" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1g7p, resolution 1.50&Aring;" />
caption="1g7p, resolution 1.50&Aring;" />
'''CRYSTAL STRUCTURE OF MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND YEAST ALPHA-GLUCOSIDASE'''<br />
'''CRYSTAL STRUCTURE OF MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND YEAST ALPHA-GLUCOSIDASE'''<br />
==Overview==
==Overview==
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The crystal structure of a non-standard peptide, YEA9, in complex with, H-2Kb, at 1.5 A resolution demonstrates how YEA9 peptide can bind with, surprisingly high affinity through insertion of alternative, long, non-canonical anchors into the B and E pockets. The use of "alternative, pockets" represents a new mode of high affinity peptide binding, that, should be considered when predicting peptide epitopes for MHC class I., These novel interactions encountered in this non-canonical high affinity, peptide-MHC complex should help predict additional binding peptides from, primary protein sequences and aid in the design of alternative approaches, for peptide-based vaccines.
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The crystal structure of a non-standard peptide, YEA9, in complex with H-2Kb, at 1.5 A resolution demonstrates how YEA9 peptide can bind with surprisingly high affinity through insertion of alternative, long, non-canonical anchors into the B and E pockets. The use of "alternative pockets" represents a new mode of high affinity peptide binding, that should be considered when predicting peptide epitopes for MHC class I. These novel interactions encountered in this non-canonical high affinity peptide-MHC complex should help predict additional binding peptides from primary protein sequences and aid in the design of alternative approaches for peptide-based vaccines.
==About this Structure==
==About this Structure==
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1G7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alpha-glucosidase Alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.20 3.2.1.20] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G7P OCA].
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1G7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alpha-glucosidase Alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.20 3.2.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7P OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Apostolopoulos, V.]]
[[Category: Apostolopoulos, V.]]
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[[Category: Corper, A.L.]]
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[[Category: Corper, A L.]]
[[Category: Li, W.]]
[[Category: Li, W.]]
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[[Category: McKenzie, I.F.]]
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[[Category: McKenzie, I F.]]
[[Category: Teyton, L.]]
[[Category: Teyton, L.]]
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[[Category: Wilson, I.A.]]
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[[Category: Wilson, I A.]]
[[Category: Yu, M.]]
[[Category: Yu, M.]]
[[Category: NAG]]
[[Category: NAG]]
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[[Category: yeast]]
[[Category: yeast]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:47:19 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:01 2008''

Revision as of 10:47, 21 February 2008


1g7p, resolution 1.50Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND YEAST ALPHA-GLUCOSIDASE

Overview

The crystal structure of a non-standard peptide, YEA9, in complex with H-2Kb, at 1.5 A resolution demonstrates how YEA9 peptide can bind with surprisingly high affinity through insertion of alternative, long, non-canonical anchors into the B and E pockets. The use of "alternative pockets" represents a new mode of high affinity peptide binding, that should be considered when predicting peptide epitopes for MHC class I. These novel interactions encountered in this non-canonical high affinity peptide-MHC complex should help predict additional binding peptides from primary protein sequences and aid in the design of alternative approaches for peptide-based vaccines.

About this Structure

1G7P is a Protein complex structure of sequences from Mus musculus with as ligand. Active as Alpha-glucosidase, with EC number 3.2.1.20 Full crystallographic information is available from OCA.

Reference

Crystal structure of a non-canonical high affinity peptide complexed with MHC class I: a novel use of alternative anchors., Apostolopoulos V, Yu M, Corper AL, Li W, McKenzie IF, Teyton L, Wilson IA, Plebanski M, J Mol Biol. 2002 May 17;318(5):1307-16. PMID:12083519

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