1gr0
From Proteopedia
(New page: 200px<br /><applet load="1gr0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gr0, resolution 1.95Å" /> '''MYO-INOSITOL 1-PHOSP...) |
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| - | [[Image:1gr0.gif|left|200px]]<br /><applet load="1gr0" size=" | + | [[Image:1gr0.gif|left|200px]]<br /><applet load="1gr0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gr0, resolution 1.95Å" /> | caption="1gr0, resolution 1.95Å" /> | ||
'''MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.'''<br /> | '''MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis | + | Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis viability and the enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents for which little structural information is available. The rate-limiting step in the pathway is the production of (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate, a complex reaction catalyzed by the enzyme inositol 1-phosphate synthase. We have determined the crystal structure of this enzyme from Mycobacterium tuberculosis (tbINO) at 1.95 A resolution, bound to the cofactor NAD+. The active site is located within a deep cleft at the junction between two domains. The unexpected presence of a zinc ion here suggests a mechanistic difference from the eukaryotic inositol synthases, which are stimulated by monovalent cations, that may be exploitable in developing selective inhibitors of tbINO. |
==About this Structure== | ==About this Structure== | ||
| - | 1GR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with ZN, CAC and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http:// | + | 1GR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CAC:'>CAC</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Mcdonald, N | + | [[Category: Mcdonald, N Q.]] |
[[Category: Murray-Rust, J.]] | [[Category: Murray-Rust, J.]] | ||
| - | [[Category: Norman, R | + | [[Category: Norman, R A.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
[[Category: CAC]] | [[Category: CAC]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:53:05 2008'' |
Revision as of 10:53, 21 February 2008
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MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.
Overview
Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis viability and the enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents for which little structural information is available. The rate-limiting step in the pathway is the production of (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate, a complex reaction catalyzed by the enzyme inositol 1-phosphate synthase. We have determined the crystal structure of this enzyme from Mycobacterium tuberculosis (tbINO) at 1.95 A resolution, bound to the cofactor NAD+. The active site is located within a deep cleft at the junction between two domains. The unexpected presence of a zinc ion here suggests a mechanistic difference from the eukaryotic inositol synthases, which are stimulated by monovalent cations, that may be exploitable in developing selective inhibitors of tbINO.
About this Structure
1GR0 is a Single protein structure of sequence from Mycobacterium tuberculosis with , and as ligands. Active as Inositol-3-phosphate synthase, with EC number 5.5.1.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis., Norman RA, McAlister MS, Murray-Rust J, Movahedzadeh F, Stoker NG, McDonald NQ, Structure. 2002 Mar;10(3):393-402. PMID:12005437
Page seeded by OCA on Thu Feb 21 12:53:05 2008
Categories: Inositol-3-phosphate synthase | Mycobacterium tuberculosis | Single protein | Mcdonald, N Q. | Murray-Rust, J. | Norman, R A. | TBSGC, TB Structural Genomics Consortium. | CAC | NAD | ZN | Oxidoreductase | Protein structure initiative | Psi | Synthase | Tb | Tb structural genomics consortium | Tbsgc
