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1guk
From Proteopedia
(New page: 200px<br /><applet load="1guk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1guk, resolution 2.9Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1guk.gif|left|200px]]<br /><applet load="1guk" size=" | + | [[Image:1guk.gif|left|200px]]<br /><applet load="1guk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1guk, resolution 2.9Å" /> | caption="1guk, resolution 2.9Å" /> | ||
'''CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4'''<br /> | '''CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes | + | Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes which play a key role in cellular detoxification. The enzymes protect the cells against toxicants by conjugating them to glutathione. Recently, a novel subgroup of alpha-class GSTs has been identified with altered substrate specificity which is particularly important for cellular defense against oxidative stress. Here, we report the crystal structure of murine GSTA4-4, which is the first structure of a prototypical member of this subgroup. The structure was solved by molecular replacement and refined to 2.9 A resolution. It resembles the structure of other members of the GST superfamily, but reveals a distinct substrate binding site. |
==About this Structure== | ==About this Structure== | ||
| - | 1GUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http:// | + | 1GUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dijkstra, B | + | [[Category: Dijkstra, B W.]] |
[[Category: Hoier, H.]] | [[Category: Hoier, H.]] | ||
[[Category: Krengel, U.]] | [[Category: Krengel, U.]] | ||
| - | [[Category: Schroter, K | + | [[Category: Schroter, K H.]] |
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: glutathione s-transferase]] | [[Category: glutathione s-transferase]] | ||
| Line 24: | Line 24: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:05 2008'' |
Revision as of 10:54, 21 February 2008
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CRYSTAL STRUCTURE OF MURINE ALPHA-CLASS GSTA4-4
Overview
Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes which play a key role in cellular detoxification. The enzymes protect the cells against toxicants by conjugating them to glutathione. Recently, a novel subgroup of alpha-class GSTs has been identified with altered substrate specificity which is particularly important for cellular defense against oxidative stress. Here, we report the crystal structure of murine GSTA4-4, which is the first structure of a prototypical member of this subgroup. The structure was solved by molecular replacement and refined to 2.9 A resolution. It resembles the structure of other members of the GST superfamily, but reveals a distinct substrate binding site.
About this Structure
1GUK is a Single protein structure of sequence from Mus musculus. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Crystal structure of a murine alpha-class glutathione S-transferase involved in cellular defense against oxidative stress., Krengel U, Schroter KH, Hoier H, Arkema A, Kalk KH, Zimniak P, Dijkstra BW, FEBS Lett. 1998 Feb 6;422(3):285-90. PMID:9498801
Page seeded by OCA on Thu Feb 21 12:54:05 2008
