1h4u
From Proteopedia
(New page: 200px<br /><applet load="1h4u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4u, resolution 2.20Å" /> '''DOMAIN G2 OF MOUSE N...) |
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| - | [[Image:1h4u.gif|left|200px]]<br /><applet load="1h4u" size=" | + | [[Image:1h4u.gif|left|200px]]<br /><applet load="1h4u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h4u, resolution 2.20Å" /> | caption="1h4u, resolution 2.20Å" /> | ||
'''DOMAIN G2 OF MOUSE NIDOGEN-1'''<br /> | '''DOMAIN G2 OF MOUSE NIDOGEN-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nidogen, an invariant component of basement membranes, is a | + | Nidogen, an invariant component of basement membranes, is a multifunctional protein that interacts with most other major basement membrane proteins. Here, we report the crystal structure of the mouse nidogen-1 G2 fragment, which contains binding sites for collagen IV and perlecan. The structure is composed of an EGF-like domain and an 11-stranded beta-barrel with a central helix. The beta-barrel domain has unexpected similarity to green fluorescent protein. A large surface patch on the beta-barrel is strikingly conserved in all metazoan nidogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1H4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1H4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: extracellular matrix protein]] | [[Category: extracellular matrix protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:22 2008'' |
Revision as of 10:57, 21 February 2008
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DOMAIN G2 OF MOUSE NIDOGEN-1
Overview
Nidogen, an invariant component of basement membranes, is a multifunctional protein that interacts with most other major basement membrane proteins. Here, we report the crystal structure of the mouse nidogen-1 G2 fragment, which contains binding sites for collagen IV and perlecan. The structure is composed of an EGF-like domain and an 11-stranded beta-barrel with a central helix. The beta-barrel domain has unexpected similarity to green fluorescent protein. A large surface patch on the beta-barrel is strikingly conserved in all metazoan nidogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding.
About this Structure
1H4U is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1., Hopf M, Gohring W, Ries A, Timpl R, Hohenester E, Nat Struct Biol. 2001 Jul;8(7):634-40. PMID:11427896
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