1h7d
From Proteopedia
(New page: 200px<br /><applet load="1h7d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h7d" /> '''SOLUTION STRUCTURE OF THE 49 AA PRESEQUENCE ...) |
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'''SOLUTION STRUCTURE OF THE 49 AA PRESEQUENCE OF 5-ALAS'''<br /> | '''SOLUTION STRUCTURE OF THE 49 AA PRESEQUENCE OF 5-ALAS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The mitochondrial import of 5-aminolevulinate synthase (ALAS), the first | + | The mitochondrial import of 5-aminolevulinate synthase (ALAS), the first enzyme of the mammalian heme biosynthetic pathway, requires the N-terminal presequence. The 49 amino acid presequence transit peptide (psALAS) for murine erythroid ALAS was chemically synthesized, and circular dichroism and (1)H nuclear magnetic resonance (NMR) spectroscopies used to determine structural elements in trifluoroethanol/H(2)O solutions and micellar environments. A well defined amphipathic alpha-helix, spanning L22 to F33, was present in psALAS in 50% trifluoroethanol. Further, a short alpha-helix, defined by A5-L8, was also apparent in the 26 amino acid N-terminus peptide, when its structure was determined in sodium dodecyl sulfate. Heme inhibition of ALAS mitochondrial import has been reported to be mediated through cysteine residues in presequence heme regulatory motifs (HRMs). A UV/visible and (1)H NMR study of hemin and psALAS indicated that a heme-peptide interaction occurs and demonstrates, for the first time, that heme interacts with the HRMs of psALAS. |
==About this Structure== | ==About this Structure== | ||
| - | 1H7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/5-aminolevulinate_synthase 5-aminolevulinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.37 2.3.1.37] Full crystallographic information is available from [http:// | + | 1H7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/5-aminolevulinate_synthase 5-aminolevulinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.37 2.3.1.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H7D OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dias, J | + | [[Category: Dias, J S.]] |
| - | [[Category: Ferreira, G | + | [[Category: Ferreira, G C.]] |
| - | [[Category: Goodfellow, B | + | [[Category: Goodfellow, B J.]] |
[[Category: Henklein, P.]] | [[Category: Henklein, P.]] | ||
| - | [[Category: Macedo, A | + | [[Category: Macedo, A L.]] |
[[Category: Wray, V.]] | [[Category: Wray, V.]] | ||
[[Category: alas]] | [[Category: alas]] | ||
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[[Category: presequence]] | [[Category: presequence]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:58:12 2008'' |
Revision as of 10:58, 21 February 2008
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SOLUTION STRUCTURE OF THE 49 AA PRESEQUENCE OF 5-ALAS
Overview
The mitochondrial import of 5-aminolevulinate synthase (ALAS), the first enzyme of the mammalian heme biosynthetic pathway, requires the N-terminal presequence. The 49 amino acid presequence transit peptide (psALAS) for murine erythroid ALAS was chemically synthesized, and circular dichroism and (1)H nuclear magnetic resonance (NMR) spectroscopies used to determine structural elements in trifluoroethanol/H(2)O solutions and micellar environments. A well defined amphipathic alpha-helix, spanning L22 to F33, was present in psALAS in 50% trifluoroethanol. Further, a short alpha-helix, defined by A5-L8, was also apparent in the 26 amino acid N-terminus peptide, when its structure was determined in sodium dodecyl sulfate. Heme inhibition of ALAS mitochondrial import has been reported to be mediated through cysteine residues in presequence heme regulatory motifs (HRMs). A UV/visible and (1)H NMR study of hemin and psALAS indicated that a heme-peptide interaction occurs and demonstrates, for the first time, that heme interacts with the HRMs of psALAS.
About this Structure
1H7D is a Single protein structure of sequence from Mus musculus. Active as 5-aminolevulinate synthase, with EC number 2.3.1.37 Full crystallographic information is available from OCA.
Reference
The solution structure and heme binding of the presequence of murine 5-aminolevulinate synthase., Goodfellow BJ, Dias JS, Ferreira GC, Henklein P, Wray V, Macedo AL, FEBS Lett. 2001 Sep 14;505(2):325-31. PMID:11566198
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