1i7e
From Proteopedia
(New page: 200px<br /><applet load="1i7e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i7e, resolution 1.95Å" /> '''C-Terminal Domain Of...) |
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| - | [[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size=" | + | [[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1i7e, resolution 1.95Å" /> | caption="1i7e, resolution 1.95Å" /> | ||
'''C-Terminal Domain Of Mouse Brain Tubby Protein bound to Phosphatidylinositol 4,5-bis-phosphate'''<br /> | '''C-Terminal Domain Of Mouse Brain Tubby Protein bound to Phosphatidylinositol 4,5-bis-phosphate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Dysfunction of the tubby protein results in maturity-onset obesity in | + | Dysfunction of the tubby protein results in maturity-onset obesity in mice. Tubby has been implicated as a transcription regulator, but details of the molecular mechanism underlying its function remain unclear. Here we show that tubby functions in signal transduction from heterotrimeric GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through its carboxyl terminal "tubby domain." X-ray crystallography reveals the atomic-level basis of this interaction and implicates tubby domains as phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated activation of G protein alphaq (Galphaq) releases tubby from the plasma membrane through the action of phospholipase C-beta, triggering translocation of tubby to the cell nucleus. The localization of tubby-like protein 3 (TULP3) is similarly regulated. These data suggest that tubby proteins function as membrane-bound transcription regulators that translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of gene expression. |
==About this Structure== | ==About this Structure== | ||
| - | 1I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with IBS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=IBS:'>IBS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7E OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baird, C | + | [[Category: Baird, C L.]] |
| - | [[Category: Boggon, T | + | [[Category: Boggon, T J.]] |
[[Category: Santagata, S.]] | [[Category: Santagata, S.]] | ||
| - | [[Category: Shan, W | + | [[Category: Shan, W S.]] |
[[Category: Shapiro, L.]] | [[Category: Shapiro, L.]] | ||
[[Category: IBS]] | [[Category: IBS]] | ||
[[Category: tubby filled-barrel beta-barrel filled-beta-roll 12-stranded-beta-barrel helix-filled-barrel obesity blindness deafness phosphoinositide phosphatidylinositol]] | [[Category: tubby filled-barrel beta-barrel filled-beta-roll 12-stranded-beta-barrel helix-filled-barrel obesity blindness deafness phosphoinositide phosphatidylinositol]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:45 2008'' |
Revision as of 11:08, 21 February 2008
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C-Terminal Domain Of Mouse Brain Tubby Protein bound to Phosphatidylinositol 4,5-bis-phosphate
Overview
Dysfunction of the tubby protein results in maturity-onset obesity in mice. Tubby has been implicated as a transcription regulator, but details of the molecular mechanism underlying its function remain unclear. Here we show that tubby functions in signal transduction from heterotrimeric GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through its carboxyl terminal "tubby domain." X-ray crystallography reveals the atomic-level basis of this interaction and implicates tubby domains as phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated activation of G protein alphaq (Galphaq) releases tubby from the plasma membrane through the action of phospholipase C-beta, triggering translocation of tubby to the cell nucleus. The localization of tubby-like protein 3 (TULP3) is similarly regulated. These data suggest that tubby proteins function as membrane-bound transcription regulators that translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of gene expression.
About this Structure
1I7E is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
G-protein signaling through tubby proteins., Santagata S, Boggon TJ, Baird CL, Gomez CA, Zhao J, Shan WS, Myszka DG, Shapiro L, Science. 2001 Jun 15;292(5524):2041-50. Epub 2001 May 24. PMID:11375483
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