1iaw
From Proteopedia
(New page: 200px<br /><applet load="1iaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iaw, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1iaw.gif|left|200px]]<br /><applet load="1iaw" size=" | + | [[Image:1iaw.gif|left|200px]]<br /><applet load="1iaw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1iaw, resolution 2.4Å" /> | caption="1iaw, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA'''<br /> | '''CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase | + | NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide. |
==About this Structure== | ==About this Structure== | ||
| - | 1IAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | + | 1IAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
| - | [[Category: Colandene, J | + | [[Category: Colandene, J D.]] |
[[Category: Huai, Q.]] | [[Category: Huai, Q.]] | ||
[[Category: Ke, H.]] | [[Category: Ke, H.]] | ||
| - | [[Category: Topal, M | + | [[Category: Topal, M D.]] |
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:53 2008'' |
Revision as of 11:09, 21 February 2008
|
CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA
Overview
NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.
About this Structure
1IAW is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement., Huai Q, Colandene JD, Topal MD, Ke H, Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254
Page seeded by OCA on Thu Feb 21 13:09:53 2008
