1idp
From Proteopedia
(New page: 200px<br /><applet load="1idp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1idp, resolution 1.45Å" /> '''Crystal structure of...) |
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| - | [[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size=" | + | [[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1idp, resolution 1.45Å" /> | caption="1idp, resolution 1.45Å" /> | ||
'''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''<br /> | '''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal | + | Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K. |
==About this Structure== | ==About this Structure== | ||
| - | 1IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http:// | + | 1IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: melanine biosynthesis]] | [[Category: melanine biosynthesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:52 2008'' |
Revision as of 11:10, 21 February 2008
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Crystal structure of scytalone dehydratase F162A mutant in the unligated state
Overview
Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.
About this Structure
1IDP is a Single protein structure of sequence from Magnaporthe grisea. Active as Scytalone dehydratase, with EC number 4.2.1.94 Full crystallographic information is available from OCA.
Reference
Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:11752795
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