1ig7
From Proteopedia
(New page: 200px<br /><applet load="1ig7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ig7, resolution 2.20Å" /> '''Msx-1 Homeodomain/DN...) |
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| - | [[Image:1ig7.gif|left|200px]]<br /><applet load="1ig7" size=" | + | [[Image:1ig7.gif|left|200px]]<br /><applet load="1ig7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ig7, resolution 2.20Å" /> | caption="1ig7, resolution 2.20Å" /> | ||
'''Msx-1 Homeodomain/DNA Complex Structure'''<br /> | '''Msx-1 Homeodomain/DNA Complex Structure'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Msx-1 homeodomain protein plays a crucial role in craniofacial, limb, and nervous system development. Homeodomain DNA-binding domains are | + | The Msx-1 homeodomain protein plays a crucial role in craniofacial, limb, and nervous system development. Homeodomain DNA-binding domains are comprised of 60 amino acids that show a high degree of evolutionary conservation. We have determined the structure of the Msx-1 homeodomain complexed to DNA at 2.2 A resolution. The structure has an unusually well-ordered N-terminal arm with a unique trajectory across the minor groove of the DNA. DNA specificity conferred by bases flanking the core TAAT sequence is explained by well ordered water-mediated interactions at Q50. Most interactions seen at the TAAT sequence are typical of the interactions seen in other homeodomain structures. Comparison of the Msx-1-HD structure to all other high resolution HD-DNA complex structures indicate a remarkably well-conserved sphere of hydration between the DNA and protein in these complexes. |
==About this Structure== | ==About this Structure== | ||
| - | 1IG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1IG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Abate-Shen, C.]] | [[Category: Abate-Shen, C.]] | ||
| - | [[Category: Geiger, J | + | [[Category: Geiger, J H.]] |
[[Category: Hovde, S.]] | [[Category: Hovde, S.]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:31 2008'' |
Revision as of 11:11, 21 February 2008
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Msx-1 Homeodomain/DNA Complex Structure
Overview
The Msx-1 homeodomain protein plays a crucial role in craniofacial, limb, and nervous system development. Homeodomain DNA-binding domains are comprised of 60 amino acids that show a high degree of evolutionary conservation. We have determined the structure of the Msx-1 homeodomain complexed to DNA at 2.2 A resolution. The structure has an unusually well-ordered N-terminal arm with a unique trajectory across the minor groove of the DNA. DNA specificity conferred by bases flanking the core TAAT sequence is explained by well ordered water-mediated interactions at Q50. Most interactions seen at the TAAT sequence are typical of the interactions seen in other homeodomain structures. Comparison of the Msx-1-HD structure to all other high resolution HD-DNA complex structures indicate a remarkably well-conserved sphere of hydration between the DNA and protein in these complexes.
About this Structure
1IG7 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Msx-1 homeodomain/DNA complex., Hovde S, Abate-Shen C, Geiger JH, Biochemistry. 2001 Oct 9;40(40):12013-21. PMID:11580277
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