1irx
From Proteopedia
(New page: 200px<br /><applet load="1irx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1irx, resolution 2.6Å" /> '''Crystal structure of ...) |
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| - | [[Image:1irx.gif|left|200px]]<br /><applet load="1irx" size=" | + | [[Image:1irx.gif|left|200px]]<br /><applet load="1irx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1irx, resolution 2.6Å" /> | caption="1irx, resolution 2.6Å" /> | ||
'''Crystal structure of class I lysyl-tRNA synthetase'''<br /> | '''Crystal structure of class I lysyl-tRNA synthetase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II | + | Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of LysRS-I and LysRS-II in complex with lysine shows that both enzymes use similar strategies for substrate recognition within unrelated active site topologies. A docking model based upon the GluRS-tRNA complex suggests how LysRS-I and LysRS-II can recognize the same molecular determinants in tRNALys, as shown by biochemical results, while approaching the acceptor helix of the tRNA from opposite sides. |
==About this Structure== | ==About this Structure== | ||
| - | 1IRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] Full crystallographic information is available from [http:// | + | 1IRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ishitani, R.]] | [[Category: Ishitani, R.]] | ||
[[Category: Nureki, O.]] | [[Category: Nureki, O.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Soll, D.]] | [[Category: Soll, D.]] | ||
[[Category: Terada, T.]] | [[Category: Terada, T.]] | ||
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[[Category: zinc-binding structure]] | [[Category: zinc-binding structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:59 2008'' |
Revision as of 11:15, 21 February 2008
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Crystal structure of class I lysyl-tRNA synthetase
Overview
Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of LysRS-I and LysRS-II in complex with lysine shows that both enzymes use similar strategies for substrate recognition within unrelated active site topologies. A docking model based upon the GluRS-tRNA complex suggests how LysRS-I and LysRS-II can recognize the same molecular determinants in tRNALys, as shown by biochemical results, while approaching the acceptor helix of the tRNA from opposite sides.
About this Structure
1IRX is a Single protein structure of sequence from Pyrococcus horikoshii with as ligand. Active as Lysine--tRNA ligase, with EC number 6.1.1.6 Full crystallographic information is available from OCA.
Reference
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies., Terada T, Nureki O, Ishitani R, Ambrogelly A, Ibba M, Soll D, Yokoyama S, Nat Struct Biol. 2002 Apr;9(4):257-62. PMID:11887185
Page seeded by OCA on Thu Feb 21 13:14:59 2008
Categories: Lysine--tRNA ligase | Pyrococcus horikoshii | Single protein | Ambrogelly, A. | Ibba, M. | Ishitani, R. | Nureki, O. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Soll, D. | Terada, T. | Yokoyama, S. | ZN | Alpha-helix cage | Beta sandwitch | Riken structural genomics/proteomics initiative | Rossmann fold | Rsgi | Structural genomics | Zinc-binding structure
