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1irx

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(New page: 200px<br /><applet load="1irx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1irx, resolution 2.6&Aring;" /> '''Crystal structure of ...)
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[[Image:1irx.gif|left|200px]]<br /><applet load="1irx" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1irx.gif|left|200px]]<br /><applet load="1irx" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1irx, resolution 2.6&Aring;" />
caption="1irx, resolution 2.6&Aring;" />
'''Crystal structure of class I lysyl-tRNA synthetase'''<br />
'''Crystal structure of class I lysyl-tRNA synthetase'''<br />
==Overview==
==Overview==
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Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II, (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from, Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity, with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of, LysRS-I and LysRS-II in complex with lysine shows that both enzymes use, similar strategies for substrate recognition within unrelated active site, topologies. A docking model based upon the GluRS-tRNA complex suggests how, LysRS-I and LysRS-II can recognize the same molecular determinants in, tRNALys, as shown by biochemical results, while approaching the acceptor, helix of the tRNA from opposite sides.
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Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of LysRS-I and LysRS-II in complex with lysine shows that both enzymes use similar strategies for substrate recognition within unrelated active site topologies. A docking model based upon the GluRS-tRNA complex suggests how LysRS-I and LysRS-II can recognize the same molecular determinants in tRNALys, as shown by biochemical results, while approaching the acceptor helix of the tRNA from opposite sides.
==About this Structure==
==About this Structure==
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1IRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IRX OCA].
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1IRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRX OCA].
==Reference==
==Reference==
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[[Category: Ishitani, R.]]
[[Category: Ishitani, R.]]
[[Category: Nureki, O.]]
[[Category: Nureki, O.]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Soll, D.]]
[[Category: Soll, D.]]
[[Category: Terada, T.]]
[[Category: Terada, T.]]
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[[Category: zinc-binding structure]]
[[Category: zinc-binding structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:37:03 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:59 2008''

Revision as of 11:15, 21 February 2008

Image:1irx.gif

1irx, resolution 2.6Å

Drag the structure with the mouse to rotate

Crystal structure of class I lysyl-tRNA synthetase

Overview

Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of LysRS-I and LysRS-II in complex with lysine shows that both enzymes use similar strategies for substrate recognition within unrelated active site topologies. A docking model based upon the GluRS-tRNA complex suggests how LysRS-I and LysRS-II can recognize the same molecular determinants in tRNALys, as shown by biochemical results, while approaching the acceptor helix of the tRNA from opposite sides.

About this Structure

1IRX is a Single protein structure of sequence from Pyrococcus horikoshii with as ligand. Active as Lysine--tRNA ligase, with EC number 6.1.1.6 Full crystallographic information is available from OCA.

Reference

Functional convergence of two lysyl-tRNA synthetases with unrelated topologies., Terada T, Nureki O, Ishitani R, Ambrogelly A, Ibba M, Soll D, Yokoyama S, Nat Struct Biol. 2002 Apr;9(4):257-62. PMID:11887185

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