1is9
From Proteopedia
(New page: 200px<br /><applet load="1is9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1is9, resolution 1.03Å" /> '''Endoglucanase A from...) |
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| - | [[Image:1is9.jpg|left|200px]]<br /><applet load="1is9" size=" | + | [[Image:1is9.jpg|left|200px]]<br /><applet load="1is9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1is9, resolution 1.03Å" /> | caption="1is9, resolution 1.03Å" /> | ||
'''Endoglucanase A from Clostridium thermocellum at atomic resolution'''<br /> | '''Endoglucanase A from Clostridium thermocellum at atomic resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the endoglucanase A from Clostridium thermocellum CelA | + | The structure of the endoglucanase A from Clostridium thermocellum CelA was re-solved by three-wavelength MAD. Experimental phases were obtained in the resolution range 25-1.0 A. Various structure-solution approaches were tested in order to quantify the contribution of each wavelength. Two-wavelength MAD phasing was sufficient to obtain excellent experimental phases. SAD at the remote wavelength also resulted in interpretable maps. The three-wavelength MAD electron-density map was of excellent quality: for parts of the structure, atom types and bond types could be easily assigned. Double bonds in peptide links and side chains could be located owing to their increased electron density indicating their pi character. Comparison with a previously determined structure of CelA at 1.65 A showed that, apart from a few additional multiple conformers and differently oriented side chains, major differences occur at the protein-solvent interface. A complete additional solvent shell could be observed and the inner shells have been completed. The high accuracy of the structure allowed unambiguous assignment of the protonation state for the active-site catalytic carboxylates. |
==About this Structure== | ==About this Structure== | ||
| - | 1IS9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] with HG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http:// | + | 1IS9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IS9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Clostridium thermocellum]] | [[Category: Clostridium thermocellum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alzari, P | + | [[Category: Alzari, P M.]] |
[[Category: Costabel, M.]] | [[Category: Costabel, M.]] | ||
[[Category: Gonzalez, A.]] | [[Category: Gonzalez, A.]] | ||
| - | [[Category: Lamzin, V | + | [[Category: Lamzin, V S.]] |
| - | [[Category: Morris, R | + | [[Category: Morris, R J.]] |
[[Category: Schmidt, A.]] | [[Category: Schmidt, A.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
| Line 25: | Line 25: | ||
[[Category: hg derivative]] | [[Category: hg derivative]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:15:05 2008'' |
Revision as of 11:15, 21 February 2008
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Endoglucanase A from Clostridium thermocellum at atomic resolution
Overview
The structure of the endoglucanase A from Clostridium thermocellum CelA was re-solved by three-wavelength MAD. Experimental phases were obtained in the resolution range 25-1.0 A. Various structure-solution approaches were tested in order to quantify the contribution of each wavelength. Two-wavelength MAD phasing was sufficient to obtain excellent experimental phases. SAD at the remote wavelength also resulted in interpretable maps. The three-wavelength MAD electron-density map was of excellent quality: for parts of the structure, atom types and bond types could be easily assigned. Double bonds in peptide links and side chains could be located owing to their increased electron density indicating their pi character. Comparison with a previously determined structure of CelA at 1.65 A showed that, apart from a few additional multiple conformers and differently oriented side chains, major differences occur at the protein-solvent interface. A complete additional solvent shell could be observed and the inner shells have been completed. The high accuracy of the structure allowed unambiguous assignment of the protonation state for the active-site catalytic carboxylates.
About this Structure
1IS9 is a Single protein structure of sequence from Clostridium thermocellum with and as ligands. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Advantages of high-resolution phasing: MAD to atomic resolution., Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1433-41. Epub 2002, Aug 23. PMID:12198299
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