1ixs

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(New page: 200px<br /><applet load="1ixs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ixs, resolution 3.2&Aring;" /> '''Structure of RuvB com...)
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[[Image:1ixs.gif|left|200px]]<br /><applet load="1ixs" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ixs.gif|left|200px]]<br /><applet load="1ixs" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ixs, resolution 3.2&Aring;" />
caption="1ixs, resolution 3.2&Aring;" />
'''Structure of RuvB complexed with RuvA domain III'''<br />
'''Structure of RuvB complexed with RuvA domain III'''<br />
==Overview==
==Overview==
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We present the X-ray structure of the RuvA-RuvB complex, which plays a, crucial role in ATP-dependent branch migration. Two RuvA tetramers form, the symmetric and closed octameric shell, where four RuvA domain IIIs, spring out in the two opposite directions to be individually caught by a, single RuvB. The binding of domain III deforms the protruding beta hairpin, in the N-terminal domain of RuvB and thereby appears to induce a, functional and less symmetric RuvB hexameric ring. The model of the, RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray, structure into the averaged electron microscopic images of the RuvA-RuvB, junction, appears to be more compatible with the branch migration mode of, a fixed RuvA-RuvB interaction than with a rotational interaction mode.
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We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.
==About this Structure==
==About this Structure==
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1IXS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ANP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA].
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1IXS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA].
==Reference==
==Reference==
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[[Category: heterodimeric protein complex]]
[[Category: heterodimeric protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:45:31 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:47 2008''

Revision as of 11:16, 21 February 2008

Image:1ixs.gif

1ixs, resolution 3.2Å

Drag the structure with the mouse to rotate

Structure of RuvB complexed with RuvA domain III

Overview

We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.

About this Structure

1IXS is a Protein complex structure of sequences from Thermus thermophilus with as ligand. Active as Adenosinetriphosphatase, with EC number 3.6.1.3 Full crystallographic information is available from OCA.

Reference

Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery., Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K, Mol Cell. 2002 Sep;10(3):671-81. PMID:12408833

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