Antizyme Inhibitor

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Revision as of 14:53, 6 July 2008

Crystal structure of the Antizyme inhibitor

spinqualitylabelsall models PDB ID 3BTN.pdb Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
AzI, unpublished structure
Coordinates:	save as pdb, mmCIF, xml

Antizyme inhibitor (AzI) regulates cellular polyamine homeostasis by binding to the polyamine-induced protein, Antizyme (Az), with greater affinity than ODC. AzI is highly homologous to ornithine decarboxylase (ODC), but is not enzymatically active. In order to understand these specific characteristics of AzI and its differences from ODC, we determined the 3D structure of mouse AzI to 2.05Å resolution. Both AzI and ODC crystallize as a (one monomer in cyan and the other in blue violet). However, fewer interactions at the dimer , a smaller buried surface area, and lack of symmetry of the interactions between residues from the two monomers in the AzI structure suggest that this dimeric structure is non-physiological. In addition, the absence of residues and interactions required for binding suggest that AzI does not bind PLP. A comparison to the revealed that AzI lacks the residues participating in PLP binding. Biochemical studies confirmed the lack of PLP binding and revealed that AzI exists as a monomer in solution while ODC is dimeric. Our findings that AzI exists as a monomer and its inability to bind PLP provide two independent explanations for its lack of enzymatic activity, and suggest the basis for its enhanced affinity towards Az.

spinqualitylabelsall models PDB ID 3BTN.pdb Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Each consists of two domains: a (residues 45–280) and a modified domain (residues 8–44 and 281–435).

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Comparison of the AzI structure to that of ODC Superposition of the AzI crystallographic dimer (cyan and blue violet) and the mODC dimer (red and lime) (PDB code 7odc). Superposition of the of mAzI and mODC showing the variable loops between monomers A and B and between monomers A and B (AzI residues 355–362 and 387–401). AzI loops are in black, and ODC loops are in yellow.

Primary Reference

Shira Albeck, Orly Dym, Tamar Unger, Zohar Snapir, Zippy Bercovich and Chaim Kahana. Crystallographic and biochemical studies revealing the structural basis for antizyme inhibitor function. Protein Sci. 2008 May; 17(5): 793-802. Epub 2008 Mar 27.