1j5h
From Proteopedia
(New page: 200px<br /><applet load="1j5h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j5h" /> '''Solution Structure of Apo-Neocarzinostatin''...) |
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| - | [[Image:1j5h.jpg|left|200px]]<br /><applet load="1j5h" size=" | + | [[Image:1j5h.jpg|left|200px]]<br /><applet load="1j5h" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of Apo-Neocarzinostatin'''<br /> | '''Solution Structure of Apo-Neocarzinostatin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The natural complex Neocarzinostatin comprises a labile chromophore | + | The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin. |
==About this Structure== | ==About this Structure== | ||
| - | 1J5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http:// | + | 1J5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
[[Category: Caddick, S.]] | [[Category: Caddick, S.]] | ||
| - | [[Category: Finucane, M | + | [[Category: Finucane, M D.]] |
| - | [[Category: Muskett, F | + | [[Category: Muskett, F W.]] |
| - | [[Category: Urbaniak, M | + | [[Category: Urbaniak, M D.]] |
| - | [[Category: Woolfson, D | + | [[Category: Woolfson, D N.]] |
[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:08 2008'' |
Revision as of 11:19, 21 February 2008
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Solution Structure of Apo-Neocarzinostatin
Overview
The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.
About this Structure
1J5H is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore., Urbaniak MD, Muskett FW, Finucane MD, Caddick S, Woolfson DN, Biochemistry. 2002 Oct 1;41(39):11731-9. PMID:12269815
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