1j90
From Proteopedia
(New page: 200px<br /><applet load="1j90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j90, resolution 2.56Å" /> '''Crystal Structure of...) |
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| - | [[Image:1j90.jpg|left|200px]]<br /><applet load="1j90" size=" | + | [[Image:1j90.jpg|left|200px]]<br /><applet load="1j90" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1j90, resolution 2.56Å" /> | caption="1j90, resolution 2.56Å" /> | ||
'''Crystal Structure of Drosophila Deoxyribonucleoside Kinase'''<br /> | '''Crystal Structure of Drosophila Deoxyribonucleoside Kinase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and | + | Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and activate a number of medically important nucleoside analogs. Here we report the structure of the Drosophila deoxyribonucleoside kinase with deoxycytidine bound at the nucleoside binding site and that of the human deoxyguanosine kinase with ATP at the nucleoside substrate binding site. Compared to the human kinase, the Drosophila kinase has a wider substrate cleft, which may be responsible for the broad substrate specificity of this enzyme. The human deoxyguanosine kinase is highly specific for purine substrates; this is apparently due to the presence of Arg 118, which provides favorable hydrogen bonding interactions with the substrate. The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases. |
==About this Structure== | ==About this Structure== | ||
| - | 1J90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with SO4 and DCZ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1J90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=DCZ:'>DCZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J90 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-deoxynucleoside complex]] | [[Category: protein-deoxynucleoside complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:04 2008'' |
Revision as of 11:20, 21 February 2008
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Crystal Structure of Drosophila Deoxyribonucleoside Kinase
Overview
Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and activate a number of medically important nucleoside analogs. Here we report the structure of the Drosophila deoxyribonucleoside kinase with deoxycytidine bound at the nucleoside binding site and that of the human deoxyguanosine kinase with ATP at the nucleoside substrate binding site. Compared to the human kinase, the Drosophila kinase has a wider substrate cleft, which may be responsible for the broad substrate specificity of this enzyme. The human deoxyguanosine kinase is highly specific for purine substrates; this is apparently due to the presence of Arg 118, which provides favorable hydrogen bonding interactions with the substrate. The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases.
About this Structure
1J90 is a Single protein structure of sequence from Drosophila melanogaster with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for substrate specificities of cellular deoxyribonucleoside kinases., Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J, Munch-Petersen B, Eriksson S, Eklund H, Nat Struct Biol. 2001 Jul;8(7):616-20. PMID:11427893
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