1je5
From Proteopedia
(New page: 200px<br /><applet load="1je5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1je5, resolution 1.90Å" /> '''Crystal Structure of...) |
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| - | [[Image:1je5.jpg|left|200px]]<br /><applet load="1je5" size=" | + | [[Image:1je5.jpg|left|200px]]<br /><applet load="1je5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1je5, resolution 1.90Å" /> | caption="1je5, resolution 1.90Å" /> | ||
'''Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7'''<br /> | '''Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The gene 2.5 protein (gp2.5) of bacteriophage T7 is a single-stranded DNA | + | The gene 2.5 protein (gp2.5) of bacteriophage T7 is a single-stranded DNA (ssDNA) binding protein that has essential roles in DNA replication and recombination. In addition to binding DNA, gp2.5 physically interacts with T7 DNA polymerase and T7 primase-helicase during replication to coordinate events at the replication fork. We have determined a 1.9-A crystal structure of gp2.5 and show that it has a conserved OB-fold (oligosaccharide/oligonucleotide binding fold) that is well adapted for interactions with ssDNA. Superposition of the OB-folds of gp2.5 and other ssDNA binding proteins reveals a conserved patch of aromatic residues that stack against the bases of ssDNA in the other crystal structures, suggesting that gp2.5 binds to ssDNA in a similar manner. An acidic C-terminal extension of the gp2.5 protein, which is required for dimer formation and for interactions with the T7 DNA polymerase and the primase-helicase, appears to be flexible and may act as a switch that modulates the DNA binding affinity of gp2.5. |
==About this Structure== | ==About this Structure== | ||
| - | 1JE5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JE5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JE5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bacteriophage t7]] | [[Category: Bacteriophage t7]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ellenberger, T | + | [[Category: Ellenberger, T E.]] |
[[Category: Hollis, T.]] | [[Category: Hollis, T.]] | ||
| - | [[Category: Richardson, C | + | [[Category: Richardson, C C.]] |
| - | [[Category: Stattel, J | + | [[Category: Stattel, J M.]] |
| - | [[Category: Walther, D | + | [[Category: Walther, D S.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: ob-fold]] | [[Category: ob-fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:36 2008'' |
Revision as of 11:21, 21 February 2008
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Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7
Overview
The gene 2.5 protein (gp2.5) of bacteriophage T7 is a single-stranded DNA (ssDNA) binding protein that has essential roles in DNA replication and recombination. In addition to binding DNA, gp2.5 physically interacts with T7 DNA polymerase and T7 primase-helicase during replication to coordinate events at the replication fork. We have determined a 1.9-A crystal structure of gp2.5 and show that it has a conserved OB-fold (oligosaccharide/oligonucleotide binding fold) that is well adapted for interactions with ssDNA. Superposition of the OB-folds of gp2.5 and other ssDNA binding proteins reveals a conserved patch of aromatic residues that stack against the bases of ssDNA in the other crystal structures, suggesting that gp2.5 binds to ssDNA in a similar manner. An acidic C-terminal extension of the gp2.5 protein, which is required for dimer formation and for interactions with the T7 DNA polymerase and the primase-helicase, appears to be flexible and may act as a switch that modulates the DNA binding affinity of gp2.5.
About this Structure
1JE5 is a Single protein structure of sequence from Bacteriophage t7 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the gene 2.5 protein, a single-stranded DNA binding protein encoded by bacteriophage T7., Hollis T, Stattel JM, Walther DS, Richardson CC, Ellenberger T, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9557-62. Epub 2001 Jul 31. PMID:11481454
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