1jf9
From Proteopedia
(New page: 200px<br /><applet load="1jf9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jf9, resolution 2.Å" /> '''Crystal Structure of s...) |
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| - | [[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size=" | + | [[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jf9, resolution 2.Å" /> | caption="1jf9, resolution 2.Å" /> | ||
'''Crystal Structure of selenocysteine lyase'''<br /> | '''Crystal Structure of selenocysteine lyase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Escherichia coli NifS CsdB protein is a member of the homodimeric | + | The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites. |
==About this Structure== | ==About this Structure== | ||
| - | 1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PLP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http:// | + | 1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Selenocysteine lyase]] | [[Category: Selenocysteine lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: Lima, C | + | [[Category: Lima, C D.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
[[Category: PLP]] | [[Category: PLP]] | ||
[[Category: cysteine]] | [[Category: cysteine]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:00 2008'' |
Revision as of 11:22, 21 February 2008
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Crystal Structure of selenocysteine lyase
Overview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Selenocysteine lyase, with EC number 4.4.1.16 Full crystallographic information is available from OCA.
Reference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
Page seeded by OCA on Thu Feb 21 13:22:00 2008
Categories: Escherichia coli | Selenocysteine lyase | Single protein | Burley, S K. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | PLP | Cysteine | New york structural genomix research consortium | Nifs | Nysgxrc | Protein structure initiative | Psi | Pursulfide | Selenocysteine | Structural genomics
