1jiw
From Proteopedia
(New page: 200px<br /><applet load="1jiw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jiw, resolution 1.74Å" /> '''Crystal structure of...) |
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| - | [[Image:1jiw.jpg|left|200px]]<br /><applet load="1jiw" size=" | + | [[Image:1jiw.jpg|left|200px]]<br /><applet load="1jiw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jiw, resolution 1.74Å" /> | caption="1jiw, resolution 1.74Å" /> | ||
'''Crystal structure of the APR-APRin complex'''<br /> | '''Crystal structure of the APR-APRin complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Serralysins are a family of metalloproteases secreted by Gram-negative | + | Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes. |
==About this Structure== | ==About this Structure== | ||
| - | 1JIW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JIW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Baumann, U.]] | [[Category: Baumann, U.]] | ||
| - | [[Category: Feltzer, R | + | [[Category: Feltzer, R E.]] |
| - | [[Category: Gray, R | + | [[Category: Gray, R D.]] |
[[Category: Hege, T.]] | [[Category: Hege, T.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: pseudomonas aeruginosa alkaline protease inhibitor]] | [[Category: pseudomonas aeruginosa alkaline protease inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:09 2008'' |
Revision as of 11:23, 21 February 2008
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Crystal structure of the APR-APRin complex
Overview
Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
About this Structure
1JIW is a Protein complex structure of sequences from Pseudomonas aeruginosa with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573
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