1jof
From Proteopedia
(New page: 200px<br /><applet load="1jof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jof, resolution 2.5Å" /> '''Neurospora crassa 3-c...) |
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| - | [[Image:1jof.jpg|left|200px]]<br /><applet load="1jof" size=" | + | [[Image:1jof.jpg|left|200px]]<br /><applet load="1jof" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jof, resolution 2.5Å" /> | caption="1jof, resolution 2.5Å" /> | ||
'''Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme'''<br /> | '''Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to | + | Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to muconolactones in microbes as part of the beta-ketoadipate pathway; some also dehalogenate muconate derivatives of xenobiotic haloaromatics. There are three different MLE classes unrelated by evolution. We present the X-ray structure of a eukaryotic MLE, Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme (NcCMLE) at 2.5 A resolution, with a seven-bladed beta propeller fold. It is related neither to bacterial MLEs nor to other beta propeller enzymes, but is structurally similar to the G protein beta subunit. It reveals a novel metal-independent cycloisomerase motif unlike the bacterial metal cofactor MLEs. Together, the bacterial MLEs and NcCMLE structures comprise a striking structural example of functional convergence in enzymes for 1,2-addition-elimination of carboxylic acids. NcCMLE and bacterial MLEs may enhance the reaction rate differently: the former by electrophilic catalysis and the latter by electrostatic stabilization of the enolate. |
==About this Structure== | ==About this Structure== | ||
| - | 1JOF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa] with SO4, PIN and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carboxy-cis,cis-muconate_cyclase Carboxy-cis,cis-muconate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.5 5.5.1.5] Full crystallographic information is available from [http:// | + | 1JOF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PIN:'>PIN</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carboxy-cis,cis-muconate_cyclase Carboxy-cis,cis-muconate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.5 5.5.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Deacon, A | + | [[Category: Deacon, A M.]] |
[[Category: Goldman, A.]] | [[Category: Goldman, A.]] | ||
[[Category: Kajander, T.]] | [[Category: Kajander, T.]] | ||
| - | [[Category: Kozarich, J | + | [[Category: Kozarich, J W.]] |
[[Category: Mazur, P.]] | [[Category: Mazur, P.]] | ||
| - | [[Category: Merckel, M | + | [[Category: Merckel, M C.]] |
[[Category: Thompson, A.]] | [[Category: Thompson, A.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: semet-protein]] | [[Category: semet-protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:52 2008'' |
Revision as of 11:24, 21 February 2008
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Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme
Overview
Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to muconolactones in microbes as part of the beta-ketoadipate pathway; some also dehalogenate muconate derivatives of xenobiotic haloaromatics. There are three different MLE classes unrelated by evolution. We present the X-ray structure of a eukaryotic MLE, Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme (NcCMLE) at 2.5 A resolution, with a seven-bladed beta propeller fold. It is related neither to bacterial MLEs nor to other beta propeller enzymes, but is structurally similar to the G protein beta subunit. It reveals a novel metal-independent cycloisomerase motif unlike the bacterial metal cofactor MLEs. Together, the bacterial MLEs and NcCMLE structures comprise a striking structural example of functional convergence in enzymes for 1,2-addition-elimination of carboxylic acids. NcCMLE and bacterial MLEs may enhance the reaction rate differently: the former by electrophilic catalysis and the latter by electrostatic stabilization of the enolate.
About this Structure
1JOF is a Single protein structure of sequence from Neurospora crassa with , and as ligands. Active as Carboxy-cis,cis-muconate cyclase, with EC number 5.5.1.5 Full crystallographic information is available from OCA.
Reference
The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:11937053
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