1jqs
From Proteopedia
(New page: 200px<br /><applet load="1jqs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jqs" /> '''Fitting of L11 protein and elongation factor...) |
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| - | [[Image:1jqs.gif|left|200px]]<br /><applet load="1jqs" size=" | + | [[Image:1jqs.gif|left|200px]]<br /><applet load="1jqs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jqs" /> | caption="1jqs" /> | ||
'''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog'''<br /> | '''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog'''<br /> | ||
==Overview== | ==Overview== | ||
| - | L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit | + | L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation. |
==About this Structure== | ==About this Structure== | ||
| - | 1JQS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1JQS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Agrawal, R | + | [[Category: Agrawal, R K.]] |
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Linde, J.]] | [[Category: Linde, J.]] | ||
| - | [[Category: Nierhaus, K | + | [[Category: Nierhaus, K H.]] |
[[Category: Segupta, J.]] | [[Category: Segupta, J.]] | ||
| - | [[Category: 70s e | + | [[Category: 70s e coli ribosome]] |
[[Category: cryo-em]] | [[Category: cryo-em]] | ||
[[Category: ef-g]] | [[Category: ef-g]] | ||
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[[Category: l11]] | [[Category: l11]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:46 2008'' |
Revision as of 11:25, 21 February 2008
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Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog
Overview
L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.
About this Structure
1JQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530
Page seeded by OCA on Thu Feb 21 13:25:46 2008
Categories: Escherichia coli | Protein complex | Agrawal, R K. | Frank, J. | Linde, J. | Nierhaus, K H. | Segupta, J. | 70s e coli ribosome | Cryo-em | Ef-g | Gtp state | L11
