1js2
From Proteopedia
(New page: 200px<br /><applet load="1js2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1js2, resolution 1.90Å" /> '''Crystal structure of...) |
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| - | [[Image:1js2.gif|left|200px]]<br /><applet load="1js2" size=" | + | [[Image:1js2.gif|left|200px]]<br /><applet load="1js2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1js2, resolution 1.90Å" /> | caption="1js2, resolution 1.90Å" /> | ||
'''Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster'''<br /> | '''Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Chromatium vinosum C77S HiPIP has been determined | + | The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation. |
==About this Structure== | ==About this Structure== | ||
| - | 1JS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Allochromatium_vinosum Allochromatium vinosum] with SF4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Allochromatium_vinosum Allochromatium vinosum] with <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Allochromatium vinosum]] | [[Category: Allochromatium vinosum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cowan, J | + | [[Category: Cowan, J A.]] |
[[Category: Hemann, C.]] | [[Category: Hemann, C.]] | ||
[[Category: Hille, R.]] | [[Category: Hille, R.]] | ||
| - | [[Category: Mansy, S | + | [[Category: Mansy, S S.]] |
[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
[[Category: Xiong, Y.]] | [[Category: Xiong, Y.]] | ||
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[[Category: serine ligated [4fe-4s] cluster]] | [[Category: serine ligated [4fe-4s] cluster]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:06 2008'' |
Revision as of 11:26, 21 February 2008
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Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster
Overview
The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.
About this Structure
1JS2 is a Single protein structure of sequence from Allochromatium vinosum with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:11802718[[Category: serine ligated [4fe-4s] cluster]]
Page seeded by OCA on Thu Feb 21 13:26:06 2008
