1jx6
From Proteopedia
(New page: 200px<br /><applet load="1jx6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jx6, resolution 1.50Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1jx6.gif|left|200px]]<br /><applet load="1jx6" size=" | + | [[Image:1jx6.gif|left|200px]]<br /><applet load="1jx6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jx6, resolution 1.50Å" /> | caption="1jx6, resolution 1.50Å" /> | ||
'''CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2'''<br /> | '''CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cell-cell communication in bacteria is accomplished through the exchange | + | Cell-cell communication in bacteria is accomplished through the exchange of extracellular signalling molecules called autoinducers. This process, termed quorum sensing, allows bacterial populations to coordinate gene expression. Community cooperation probably enhances the effectiveness of processes such as bioluminescence, virulence factor expression, antibiotic production and biofilm development. Unlike other autoinducers, which are specific to a particular species of bacteria, a recently discovered autoinducer (AI-2) is produced by a large number of bacterial species. AI-2 has been proposed to serve as a 'universal' signal for inter-species communication. The chemical identity of AI-2 has, however, proved elusive. Here we present the crystal structure of an AI-2 sensor protein, LuxP, in a complex with autoinducer. The bound ligand is a furanosyl borate diester that bears no resemblance to previously characterized autoinducers. Our findings suggest that addition of naturally occurring borate to an AI-2 precursor generates active AI-2. Furthermore, they indicate a potential biological role for boron, an element required by a number of organisms but for unknown reasons. |
==About this Structure== | ==About this Structure== | ||
| - | 1JX6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with CA and AI2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JX6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=AI2:'>AI2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JX6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio harveyi]] | [[Category: Vibrio harveyi]] | ||
| - | [[Category: BassleR, B | + | [[Category: BassleR, B L.]] |
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
| - | [[Category: Dorsselaer, A | + | [[Category: Dorsselaer, A Van.]] |
| - | [[Category: Hughson, F | + | [[Category: Hughson, F M.]] |
[[Category: Pelczer, I.]] | [[Category: Pelczer, I.]] | ||
[[Category: Potier, N.]] | [[Category: Potier, N.]] | ||
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[[Category: protein-ligand complex]] | [[Category: protein-ligand complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:42 2008'' |
Revision as of 11:27, 21 February 2008
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CRYSTAL STRUCTURE OF LUXP FROM VIBRIO HARVEYI COMPLEXED WITH AUTOINDUCER-2
Overview
Cell-cell communication in bacteria is accomplished through the exchange of extracellular signalling molecules called autoinducers. This process, termed quorum sensing, allows bacterial populations to coordinate gene expression. Community cooperation probably enhances the effectiveness of processes such as bioluminescence, virulence factor expression, antibiotic production and biofilm development. Unlike other autoinducers, which are specific to a particular species of bacteria, a recently discovered autoinducer (AI-2) is produced by a large number of bacterial species. AI-2 has been proposed to serve as a 'universal' signal for inter-species communication. The chemical identity of AI-2 has, however, proved elusive. Here we present the crystal structure of an AI-2 sensor protein, LuxP, in a complex with autoinducer. The bound ligand is a furanosyl borate diester that bears no resemblance to previously characterized autoinducers. Our findings suggest that addition of naturally occurring borate to an AI-2 precursor generates active AI-2. Furthermore, they indicate a potential biological role for boron, an element required by a number of organisms but for unknown reasons.
About this Structure
1JX6 is a Single protein structure of sequence from Vibrio harveyi with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural identification of a bacterial quorum-sensing signal containing boron., Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, Bassler BL, Hughson FM, Nature. 2002 Jan 31;415(6871):545-9. PMID:11823863
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