1jxg
From Proteopedia
(New page: 200px<br /><applet load="1jxg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jxg, resolution 1.6Å" /> '''The 1.6 A Resolution ...) |
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| - | [[Image:1jxg.gif|left|200px]]<br /><applet load="1jxg" size=" | + | [[Image:1jxg.gif|left|200px]]<br /><applet load="1jxg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jxg, resolution 1.6Å" /> | caption="1jxg, resolution 1.6Å" /> | ||
'''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge'''<br /> | '''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Plastocyanin is an electron-transfer protein which has been largely used | + | Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit. |
==About this Structure== | ==About this Structure== | ||
| - | 1JXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with CU1 and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with <scene name='pdbligand=CU1:'>CU1</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cannistraro, S.]] | [[Category: Cannistraro, S.]] | ||
[[Category: Milani, M.]] | [[Category: Milani, M.]] | ||
| - | [[Category: Verbeet, M | + | [[Category: Verbeet, M P.]] |
[[Category: CU1]] | [[Category: CU1]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:48 2008'' |
Revision as of 11:27, 21 February 2008
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The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge
Overview
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
About this Structure
1JXG is a Single protein structure of sequence from Populus nigra with and as ligands. Full crystallographic information is available from OCA.
Reference
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761
Page seeded by OCA on Thu Feb 21 13:27:48 2008
Categories: Populus nigra | Single protein | Andolfi, L. | Bolognesi, M. | Cannistraro, S. | Milani, M. | Verbeet, M P. | CU1 | SO4 | Beta barrel
