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1unb
From Proteopedia
(New page: 200px<br /> <applet load="1unb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1unb, resolution 1.5Å" /> '''DEACETOXYCEPHALOSPOR...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1UNB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE2 and AKG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UNB OCA]]. | + | 1UNB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE2 and AKG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Deacetoxycephalosporin-C_synthase Deacetoxycephalosporin-C synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UNB OCA]]. |
==Reference== | ==Reference== | ||
The structural basis of cephalosporin formation in a mononuclear ferrous enzyme., Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I, Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14718929 14718929] | The structural basis of cephalosporin formation in a mononuclear ferrous enzyme., Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I, Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14718929 14718929] | ||
| + | [[Category: Deacetoxycephalosporin-C synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces clavuligerus]] | [[Category: Streptomyces clavuligerus]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:06:47 2007'' |
Revision as of 11:02, 30 October 2007
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DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH 2-OXOGLUTARATE AND AMPICILLIN
Overview
Deacetoxycephalosporin-C synthase (DAOCS) is a mononuclear ferrous enzyme, that transforms penicillins into cephalosporins by inserting a carbon atom, into the penicillin nucleus. In the first half-reaction, dioxygen and, 2-oxoglutarate produce a reactive iron-oxygen species, succinate and CO2., The oxidizing iron species subsequently reacts with penicillin to give, cephalosporin and water. Here we describe high-resolution structures for, ferrous DAOCS in complex with penicillins, the cephalosporin product, the, cosubstrate and the coproduct. Steady-state kinetic data, quantum-chemical, calculations and the new structures indicate a reaction sequence in which, a 'booby-trapped' oxidizing species is formed. This species is stabilized, by the negative charge of succinate on the iron. The ... [(full description)]
About this Structure
1UNB is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with FE2 and AKG as [ligands]. Active as [Deacetoxycephalosporin-C synthase], with EC number [1.14.20.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structural basis of cephalosporin formation in a mononuclear ferrous enzyme., Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I, Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:14718929
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