1k3f
From Proteopedia
(New page: 200px<br /><applet load="1k3f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k3f, resolution 2.5Å" /> '''Uridine Phosphorylase...) |
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| - | [[Image:1k3f.jpg|left|200px]]<br /><applet load="1k3f" size=" | + | [[Image:1k3f.jpg|left|200px]]<br /><applet load="1k3f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k3f, resolution 2.5Å" /> | caption="1k3f, resolution 2.5Å" /> | ||
'''Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice'''<br /> | '''Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Uridine phosphorylase from E. coli (Upase) has been crystallized using | + | Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments. |
==About this Structure== | ==About this Structure== | ||
| - | 1K3F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http:// | + | 1K3F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Uridine phosphorylase]] | [[Category: Uridine phosphorylase]] | ||
| - | [[Category: Armstrong, S | + | [[Category: Armstrong, S R.]] |
| - | [[Category: Blagova, E | + | [[Category: Blagova, E V.]] |
| - | [[Category: Burlakova, A | + | [[Category: Burlakova, A A.]] |
| - | [[Category: Debabov, V | + | [[Category: Debabov, V G.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
| - | [[Category: Komissarov, A | + | [[Category: Komissarov, A A.]] |
| - | [[Category: Linkova, E | + | [[Category: Linkova, E V.]] |
[[Category: Mao, C.]] | [[Category: Mao, C.]] | ||
| - | [[Category: Mikhailov, A | + | [[Category: Mikhailov, A M.]] |
| - | [[Category: Mironov, A | + | [[Category: Mironov, A S.]] |
| - | [[Category: Morgunova, E | + | [[Category: Morgunova, E Yu.]] |
| - | [[Category: Popov, A | + | [[Category: Popov, A N.]] |
| - | [[Category: Smirnova, E | + | [[Category: Smirnova, E A.]] |
| - | [[Category: Vainshtein, B | + | [[Category: Vainshtein, B K.]] |
[[Category: hexamer]] | [[Category: hexamer]] | ||
[[Category: nucleoside phosphorylase]] | [[Category: nucleoside phosphorylase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:39 2008'' |
Revision as of 11:29, 21 February 2008
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Uridine Phosphorylase from E. coli, Refined in the Monoclinic Crystal Lattice
Overview
Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments.
About this Structure
1K3F is a Single protein structure of sequence from Escherichia coli. Active as Uridine phosphorylase, with EC number 2.4.2.3 Full crystallographic information is available from OCA.
Reference
Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice., Morgunova EYu, Mikhailov AM, Popov AN, Blagova EV, Smirnova EA, Vainshtein BK, Mao C, Armstrong ShR, Ealick SE, Komissarov AA, et al., FEBS Lett. 1995 Jun 26;367(2):183-7. PMID:7796917
Page seeded by OCA on Thu Feb 21 13:29:39 2008
Categories: Escherichia coli | Single protein | Uridine phosphorylase | Armstrong, S R. | Blagova, E V. | Burlakova, A A. | Debabov, V G. | Ealick, S E. | Komissarov, A A. | Linkova, E V. | Mao, C. | Mikhailov, A M. | Mironov, A S. | Morgunova, E Yu. | Popov, A N. | Smirnova, E A. | Vainshtein, B K. | Hexamer | Nucleoside phosphorylase
