1czi
From Proteopedia
(New page: 200px<br /> <applet load="1czi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1czi, resolution 2.30Å" /> '''CHYMOSIN COMPLEX WI...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1CZI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.4 3.4.23.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZI OCA]]. | + | 1CZI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/Chymosin Chymosin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.4 3.4.23.4]]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZI OCA]]. |
==Reference== | ==Reference== | ||
A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure., Groves MR, Dhanaraj V, Badasso M, Nugent P, Pitts JE, Hoover DJ, Blundell TL, Protein Eng. 1998 Oct;11(10):833-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9862200 9862200] | A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure., Groves MR, Dhanaraj V, Badasso M, Nugent P, Pitts JE, Hoover DJ, Blundell TL, Protein Eng. 1998 Oct;11(10):833-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9862200 9862200] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| + | [[Category: Chymosin]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Badasso, M.]] | [[Category: Badasso, M.]] | ||
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[[Category: inhibitor complex]] | [[Category: inhibitor complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:07:27 2007'' |
Revision as of 11:02, 30 October 2007
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CHYMOSIN COMPLEX WITH THE INHIBITOR CP-113972
Overview
In the crystal structure of uncomplexed native chymosin, the beta-hairpin, at the active site, known as 'the flap', adopts a different conformation, from that of other aspartic proteinases. This conformation would prevent, the mode of binding of substrates/inhibitors generally found in other, aspartic proteinase complexes. We now report the X-ray analysis of, chymosin complexed with a reduced bond inhibitor CP-113972 inverted, question mark(2R,3S)-isopropyl, 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexy, l-2-hydroxybutanoate inverted question mark at 2.3 A resolution in a novel, crystal form of spacegroup R32. The structure has been refined by, restrained least-squares methods to a final R-factor of 0.19 for a total, of 11 988 independent reflections in the ... [(full description)]
About this Structure
1CZI is a [Single protein] structure of sequence from [Bos taurus]. Active as [Chymosin], with EC number [3.4.23.4]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [OCA].
Reference
A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure., Groves MR, Dhanaraj V, Badasso M, Nugent P, Pitts JE, Hoover DJ, Blundell TL, Protein Eng. 1998 Oct;11(10):833-40. PMID:9862200
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