1kd1
From Proteopedia
(New page: 200px<br /><applet load="1kd1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kd1, resolution 3.00Å" /> '''Co-crystal Structure...) |
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| - | [[Image:1kd1.gif|left|200px]]<br /><applet load="1kd1" size=" | + | [[Image:1kd1.gif|left|200px]]<br /><applet load="1kd1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kd1, resolution 3.00Å" /> | caption="1kd1, resolution 3.00Å" /> | ||
'''Co-crystal Structure of Spiramycin bound to the 50S Ribosomal Subunit of Haloarcula marismortui'''<br /> | '''Co-crystal Structure of Spiramycin bound to the 50S Ribosomal Subunit of Haloarcula marismortui'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystal structures of the Haloarcula marismortui large ribosomal subunit | + | Crystal structures of the Haloarcula marismortui large ribosomal subunit complexed with the 16-membered macrolide antibiotics carbomycin A, spiramycin, and tylosin and a 15-membered macrolide, azithromycin, show that they bind in the polypeptide exit tunnel adjacent to the peptidyl transferase center. Their location suggests that they inhibit protein synthesis by blocking the egress of nascent polypeptides. The saccharide branch attached to C5 of the lactone rings extends toward the peptidyl transferase center, and the isobutyrate extension of the carbomycin A disaccharide overlaps the A-site. Unexpectedly, a reversible covalent bond forms between the ethylaldehyde substituent at the C6 position of the 16-membered macrolides and the N6 of A2103 (A2062, E. coli). Mutations in 23S rRNA that result in clinical resistance render the binding site less complementary to macrolides. |
==About this Structure== | ==About this Structure== | ||
| - | 1KD1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with SPR, MG, NA, CD, CL and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1KD1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=SPR:'>SPR</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CD:'>CD</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KD1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ban, N.]] | [[Category: Ban, N.]] | ||
| - | [[Category: Hansen, J | + | [[Category: Hansen, J L.]] |
| - | [[Category: Ippolito, J | + | [[Category: Ippolito, J A.]] |
| - | [[Category: Moore, P | + | [[Category: Moore, P B.]] |
[[Category: Nissen, P.]] | [[Category: Nissen, P.]] | ||
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: spiramycin]] | [[Category: spiramycin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:42 2008'' |
Revision as of 11:32, 21 February 2008
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Co-crystal Structure of Spiramycin bound to the 50S Ribosomal Subunit of Haloarcula marismortui
Overview
Crystal structures of the Haloarcula marismortui large ribosomal subunit complexed with the 16-membered macrolide antibiotics carbomycin A, spiramycin, and tylosin and a 15-membered macrolide, azithromycin, show that they bind in the polypeptide exit tunnel adjacent to the peptidyl transferase center. Their location suggests that they inhibit protein synthesis by blocking the egress of nascent polypeptides. The saccharide branch attached to C5 of the lactone rings extends toward the peptidyl transferase center, and the isobutyrate extension of the carbomycin A disaccharide overlaps the A-site. Unexpectedly, a reversible covalent bond forms between the ethylaldehyde substituent at the C6 position of the 16-membered macrolides and the N6 of A2103 (A2062, E. coli). Mutations in 23S rRNA that result in clinical resistance render the binding site less complementary to macrolides.
About this Structure
1KD1 is a Protein complex structure of sequences from Haloarcula marismortui with , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The structures of four macrolide antibiotics bound to the large ribosomal subunit., Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA, Mol Cell. 2002 Jul;10(1):117-28. PMID:12150912
Page seeded by OCA on Thu Feb 21 13:32:42 2008
Categories: Haloarcula marismortui | Protein complex | Ban, N. | Hansen, J L. | Ippolito, J A. | Moore, P B. | Nissen, P. | Steitz, T A. | CD | CL | K | MG | NA | SPR | 50s subunit | Antibiotic | Carbinolamine | Macrolide | Spiramycin
