1kf8
From Proteopedia
(New page: 200px<br /><applet load="1kf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kf8, resolution 1.15Å" /> '''Atomic resolution st...) |
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| - | [[Image:1kf8.jpg|left|200px]]<br /><applet load="1kf8" size=" | + | [[Image:1kf8.jpg|left|200px]]<br /><applet load="1kf8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kf8, resolution 1.15Å" /> | caption="1kf8, resolution 1.15Å" /> | ||
'''Atomic resolution structure of RNase A at pH 8.8'''<br /> | '''Atomic resolution structure of RNase A at pH 8.8'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The diffraction pattern of protein crystals extending to atomic resolution | + | The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A. |
==About this Structure== | ==About this Structure== | ||
| - | 1KF8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | + | 1KF8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KF8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Berisio, R.]] | [[Category: Berisio, R.]] | ||
| - | [[Category: Lamzin, V | + | [[Category: Lamzin, V S.]] |
[[Category: Mazzarella, L.]] | [[Category: Mazzarella, L.]] | ||
[[Category: Sica, F.]] | [[Category: Sica, F.]] | ||
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: Zagari, A.]] | [[Category: Zagari, A.]] | ||
[[Category: crystal]] | [[Category: crystal]] | ||
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[[Category: titration]] | [[Category: titration]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:33:28 2008'' |
Revision as of 11:33, 21 February 2008
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Atomic resolution structure of RNase A at pH 8.8
Overview
The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
About this Structure
1KF8 is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829
Page seeded by OCA on Thu Feb 21 13:33:28 2008
Categories: Bos taurus | Pancreatic ribonuclease | Single protein | Berisio, R. | Lamzin, V S. | Mazzarella, L. | Sica, F. | Wilson, K S. | Zagari, A. | Crystal | Ph | Rnase a | Soaking | Titration
